Metabolic Profiling of Human Eosinophils.

Frontiers in Immunology
Linsey PorterEdwin R Chilvers

Abstract

Immune cells face constant changes in their microenvironment, which requires rapid metabolic adaptation. In contrast to neutrophils, which are known to rely near exclusively on glycolysis, the metabolic profile of human eosinophils has not been characterized. Here, we assess the key metabolic parameters of peripheral blood-derived human eosinophils using real-time extracellular flux analysis to measure extracellular acidification rate and oxygen consumption rate, and compare these parameters to human neutrophils. Using this methodology, we demonstrate that eosinophils and neutrophils have a similar glycolytic capacity, albeit with a minimal glycolytic reserve. However, compared to neutrophils, eosinophils exhibit significantly greater basal mitochondrial respiration, ATP-linked respiration, maximum respiratory capacity, and spare respiratory capacity. Of note, the glucose oxidation pathway is also utilized by eosinophils, something not evident in neutrophils. Furthermore, using a colorimetric enzymatic assay, we show that eosinophils have much reduced glycogen stores compared to neutrophils. We also show that physiologically relevant levels of hypoxia (PO2 3 kPa), by suppressing oxygen consumption rates, have a profound effect ...Continue Reading

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Citations

Jan 10, 2020·Biology of the Cell·Kathleen R BashantEdwin R Chilvers
May 10, 2020·Journal of Leukocyte Biology·Kim S LeMessurierAmali E Samarasinghe
Aug 9, 2020·Clinical and Translational Gastroenterology·Girish HiremathAnita Mahadevan-Jansen
Dec 19, 2020·Intensive Care Medicine Experimental·Xiaomin ZhangClair Hartmann
Dec 8, 2020·Frontiers in Cardiovascular Medicine·Ibrahim AlZaimAhmed F El-Yazbi
Mar 17, 2021·Journal of Leukocyte Biology·Walter David Cruz-PinedaOlga Lilia Garibay-Cerdenares
Apr 21, 2021·Current Opinion in Pharmacology·Gustavo Gastão DavanzoPedro Manoel M Moraes-Vieira
Mar 5, 2021·American Journal of Respiratory Cell and Molecular Biology·Allison M ManuelYvonne M W Janssen-Heininger
Jun 26, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Sean R HolmCatherine A Thornton
Dec 2, 2019·Journal of Innate Immunity·Charalambos MichaeloudesIan M Adcock

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Methods Mentioned

BETA
Assay
chip
light microscopy
PMA
lavage

Software Mentioned

Cell
GraphPad Prism
ShapeOut
Tak
XF Hypoxia Rate Calculator

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