Metabolic profiling of Staphylococcus aureus cultivated under aerobic and anaerobic conditions with (1)H NMR-based nontargeted analysis

Canadian Journal of Microbiology
Ji-Lu SunBei-Zhong Han

Abstract

Staphylococcus aureus is a major pathogen in the medical area and food-producing sector. Detailed analyses of its basic cell physiology will help comprehensively understand this pathogen, which will be useful for developing novel diagnostic and treatment tools. Oxygen is one of the most crucial growth-limiting factors for S. aureus. In this study, to characterize and distinguish metabolic profiles of S. aureus cultivated under aerobic and anaerobic conditions, nontargeted analyses of both types of cultures were carried out using (1)H nuclear magnetic resonance spectroscopy. Fifty compounds were identified by Chenomx software. Characteristics of metabolic profiles were achieved by using principal components analysis. During aerobic growth, S. aureus mainly consumed glucose, alanine, arginine, glycine, isoleucine, leucine, phenylalanine, and acetate. Meanwhile, it accumulated 17 metabolites, mainly 2-oxoglutarate, isobutyrate, isovalerate, succinate, and ethanol. Under anaerobic condition, S. aureus mainly consumed glucose, arginine, and threonine. Meanwhile, it accumulated 13 metabolites, mainly ethanol, lactate, and ornithine. The representative metabolites that could most significantly differentiate metabolic profiles of S. au...Continue Reading

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Citations

Nov 28, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Leónides FernándezJuan Miguel Rodríguez
Aug 15, 2015·Journal of Neurosurgery·Daniel DahlbergBjørnar Hassel
Nov 7, 2014·Metabolomics : Official Journal of the Metabolomic Society·Thomas WeissgerberChristiane Dahl
Jan 21, 2014·International Journal of Medical Microbiology : IJMM·Manuel Liebeke, Michael Lalk
Aug 11, 2016·Analytica Chimica Acta·Young-Sang JungGeum-Sook Hwang
Feb 21, 2018·Biotechnology and Bioengineering·Mia M KiamcoRyan S Renslow
Nov 18, 2015·World Journal of Gastroenterology : WJG·René FahrnerFalk Rauchfuß
Jan 1, 2021·Clinical, Cosmetic and Investigational Dermatology·Ia KhmaladzeAlain Mavon
Feb 3, 2021·Journal of Bacteriology·Anne TroitzschJan Pané-Farré
Jan 30, 2019·Environmental Science & Technology·Rebecca A HillNicholas C Fitzkee

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