Metabolic Profiling with Gas Chromatography-Mass Spectrometry and Capillary Electrophoresis-Mass Spectrometry Reveals the Carbon-Nitrogen Status of Tobacco Leaves Across Different Planting Areas

Journal of Proteome Research
Jieyu ZhaoGuowang Xu

Abstract

The interaction between carbon (C) and nitrogen (N) metabolism can reflect plant growth status and environmental factors. Little is known regarding the connections between C-N metabolism and growing regions under field conditions. To comprehensively investigate the relationship in mature tobacco leaves, we established metabolomics approaches based on gas chromatography-mass spectrometry (GC-MS) and capillary electrophoresis-time-of-flight-mass spectrometry (CE-TOF-MS). Approximately 240 polar metabolites were determined. Multivariate statistical analysis revealed that the growing region greatly influenced the metabolic profiles of tobacco leaves. A metabolic correlation network and related pathway maps were used to reveal the global overview of the alteration of C-N metabolism across three typical regions. In Yunnan, sugars and tricarboxylic acid (TCA) cycle intermediates were closely correlated with amino acid pools. Henan tobacco leaves showed positive correlation between the pentose phosphate pathway (PPP) intermediates and C-rich secondary metabolism. In Guizhou, the proline and asparagine had significant links with TCA cycle intermediates and urea cycle, and antioxidant accumulation was observed in response to drought. The...Continue Reading

References

Oct 26, 1999·Trends in Plant Science·B J ShelpM D McLean
Jan 1, 2003·Journal of Experimental Botany·Christine H FoyerGraham Noctor
May 20, 2003·Current Opinion in Plant Biology·Nicholas J Kruger, Antje von Schaewen
May 12, 2004·Current Opinion in Plant Biology·Alisdair R FernieLee J Sweetlove
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wolfram WeckwerthOliver Fiehn
Sep 15, 2005·Journal of Experimental Botany·Astrid WinglerNathalie Pourtau
Nov 4, 2006·Plant, Cell & Environment·Christina FritzMark Stitt
Nov 24, 2006·Plant Physiology·Charles J BaxterLee J Sweetlove
Dec 25, 2007·Trends in Plant Science·Aaron FaitAlisdair R Fernie
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
Jun 27, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Helen G GikaIan D Wilson
Dec 25, 2008·Electrophoresis·Rawi RamautarGerhardus J de Jong
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Jan 5, 2011·BMC Systems Biology·Atsushi FukushimaKazuki Saito
Jan 12, 2011·Journal of Experimental Botany·Miyako KusanoKazuki Saito
Jun 21, 2011·Trends in Plant Science·Wagner L AraújoAlisdair R Fernie
Sep 1, 2011·Analytical and Bioanalytical Chemistry·Bogusław Buszewski, Sylwia Noga
Jan 11, 2012·The Plant Journal : for Cell and Molecular Biology·Fumio MatsudaKazuki Saito
Feb 1, 2012·Journal of Experimental Botany·Julia Krasensky, Claudia Jonak
Feb 28, 2013·Journal of Agricultural and Food Chemistry·Li ZhangFuwei Xie
Apr 30, 2013·The Plant Journal : for Cell and Molecular Biology·John D BussellSteven M Smith
Sep 6, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jose M KneeThomas J S Merritt

❮ Previous
Next ❯

Citations

Jul 31, 2016·Analytical and Bioanalytical Chemistry·Yang ZhouGuowang Xu
Nov 1, 2016·Electrophoresis·Rawi RamautarGerhardus J de Jong
Dec 24, 2016·Mass Spectrometry Reviews·Aihua ZhangXijun Wang
Aug 15, 2017·Electrophoresis·Véréna PoinsotFrançois Couderc
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.