PMID: 15244061Jul 13, 2004Paper

Metabolic properties, stress tolerance and macromolecular profiles of rhizobia nodulating Hedysarum coronarium

Journal of Applied Microbiology
Paolo StruffiMarco P Nuti

Abstract

The drought-tolerant legume Hedysarum coronarium is a Mediterranean species valued as a forage crop for its high performance in stressful conditions. The plant shows peculiar capabilities of nodulating above pH 9 and thriving in highly calcareous soils. With the aim of providing an adequate characterization of its bacterial symbiotic partner, a study was undertaken, approaching from several viewpoints the physiology and structural features of bacteria isolated from nodules of H. coronarium. Tests involved trophic capabilities on different carbon and nitrogen sources, vitamin requirements, and resistance to factors including antibiotics, heavy metals, salinity, pH, and temperature. Enzyme activities, including those of cellulase, pectinase, urease, beta-galactosidase, nitrate and nitrite reductase, were evaluated. The DNA G + C percentage content was determined. Species-specific bacteriophages were isolated and a strain-typing grid established. In order to characterize further and fingerprint the different Rhizobium 'hedysari' isolates, electrophoretic pattern of proteins, plasmid DNA, and digested genomic DNA (in pulsed-field gel separation) were compared. Adansonian taxonomy yielded similarity clusters of the different isolates.

Citations

Dec 18, 2007·Applied and Environmental Microbiology·M Eugenia Nuñez-ValdezFrancisco J Villalobos
Apr 2, 2016·Antonie van Leeuwenhoek·Khadidja AlilicheAli Chriki
Feb 7, 2009·International Journal of Systematic and Evolutionary Microbiology·Odile BergeWafa Achouak
Jan 18, 2007·FEMS Microbiology Letters·Marina BasagliaSergio Casella
Jan 19, 2006·Biochemical Society Transactions·S CasellaM Basaglia
Jul 26, 2011·Journal of Agricultural and Food Chemistry·Olekile TibeDavid R K Harding

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