Metabolic radiolabeling and in vivo PET imaging of cytotoxic T lymphocytes to guide combination adoptive cell transfer cancer therapy.

Journal of Nanobiotechnology
Dehua LuZhaofei Liu

Abstract

Adoptive T cell transfer-based immunotherapy yields unsatisfactory results in the treatment of solid tumors, partially owing to limited tumor infiltration and the immunosuppressive microenvironment in solid tumors. Therefore, strategies for the noninvasive tracking of adoptive T cells are critical for monitoring tumor infiltration and for guiding the development of novel combination therapies. We developed a radiolabeling method for cytotoxic T lymphocytes (CTLs) that comprises metabolically labeling the cell surface glycans with azidosugars and then covalently conjugating them with 64Cu-1,4,7-triazacyclononanetriacetic acid-dibenzo-cyclooctyne (64Cu-NOTA-DBCO) using bioorthogonal chemistry. 64Cu-labeled control-CTLs and ovalbumin-specific CTLs (OVA-CTLs) were tracked using positron emission tomography (PET) in B16-OVA tumor-bearing mice. We also investigated the effects of focal adhesion kinase (FAK) inhibition on the antitumor efficacy of OVA-CTLs using a poly(lactic-co-glycolic) acid (PLGA)-encapsulated nanodrug (PLGA-FAKi). CTLs can be stably radiolabeled with 64Cu with a minimal effect on cell viability. PET imaging of 64Cu-OVA-CTLs enables noninvasive mapping of their in vivo behavior. Moreover, 64Cu-OVA-CTLs PET imaging ...Continue Reading

References

Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nona AdonaiSanjiv Sam Gambhir
Apr 19, 2003·The Journal of Biological Chemistry·Jinbin ZhaiDavid D Schlaepfer
Jan 13, 2006·Nature Chemical Biology·Jennifer A Prescher, Carolyn R Bertozzi
Jul 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mikael J PittetRalph Weissleder
Mar 15, 2008·Cancer Research·Walter Gregory RobertsFelix Vajdos
Sep 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brian A RabinovichPatrick Hwu
Nov 19, 2008·Nature Clinical Practice. Oncology·Shahriar S YaghoubiSanjiv S Gambhir
Nov 26, 2009·Current Opinion in Genetics & Development·Rachel E LaingCaius G Radu
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Pamela V ChangCarolyn R Bertozzi
Dec 15, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Hanwen ZhangPeter M Smith-Jones
Mar 23, 2012·Nature Reviews. Immunology·Nicholas P RestifoSteven A Rosenberg
Apr 27, 2012·Physiological Reviews·Michelle L James, Sanjiv S Gambhir
Sep 21, 2013·Nature·Melissa R Junttila, Frederic J de Sauvage
Sep 21, 2013·Nature Immunology·Thomas F GajewskiYang-Xin Fu
Apr 5, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Mark A CastanaresMartin G Pomper
Aug 8, 2014·Nature Reviews. Cancer·Florian J SulzmaierDavid D Schlaepfer
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christoph M GriessingerManfred Kneilling
Apr 4, 2015·Science·Steven A Rosenberg, Nicholas P Restifo
Jan 20, 2017·Science Translational Medicine·Khun Visith KeuSanjiv S Gambhir
Jan 10, 2018·Frontiers in Immunology·Hamid R MirzaeiBehnam Badie
May 8, 2018·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Michael R WeistJohn E Shively
Jun 7, 2018·Frontiers in Immunology·Paris KostiJames N Arnold
Jun 16, 2018·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Simone KrebsWolfgang A Weber
Jul 4, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jai Woong SeoKatherine W Ferrara
Aug 24, 2018·Cancer Discovery·Robbie G Majzner, Crystal L Mackall
Dec 12, 2018·Annual Review of Immunology·Sonia GuedanCarl H June
May 6, 2019·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Simone KrebsHeiko Schöder
Aug 1, 2019·The Lancet Oncology·Il MinnMartin G Pomper
Nov 30, 2019·Nature Reviews. Chemistry·Christian AgatemorKevin J Yarema
Apr 4, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Candice Ashmore-HarrisGilbert O Fruhwirth
Jun 17, 2020·Nature Biomedical Engineering·Kyung Oh JungGuillem Pratx
Jul 7, 2020·Trends in Cell Biology·Michael St Paul, Pamela S Ohashi

❮ Previous
Next ❯

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.