Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy

BioRxiv : the Preprint Server for Biology
A. H. ClowsleyChristian Soeller


DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA 'imagers' to target epitopes. Its performance in biological samples is often constrained by spurious signals produced by excess imagers. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.

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