PMID: 2121717Nov 1, 1990Paper

Metabolic relationships between pyridoxine (vitamin B6) and serine biosynthesis in Escherichia coli K-12

Journal of Bacteriology
H M Lam, M E Winkler

Abstract

We propose a pathway leading from erythrose-4-phosphate and glutamate to nitrogen 1 and carbons 5,5', and 6 of the pyridoxine ring. This pathway, which parallels the phosphorylated pathway of serine biosynthesis, is predicted on the homology between PdxB and SerA, the structural similarity between serine and 4-hydroxythreonine, and the possible involvement of SerC in pyridoxine biosynthesis. Several predictions of this hypothetical scheme were tested. Consistent with the proposed pathway, supplement inhibition patterns strongly suggest that SerA enzyme acts in a an alternate pathway of pyridoxine biosynthesis in pdxB mutants. Direct enzyme assays detected erythrose-4-phosphate dehydrogenase activity in crude extracts, which again supports the proposed pathway. Chromosomal insertions in serC caused a requirement for pyridoxine, serine, and aromatic compounds, which directly verified that SerC functions in the pyridoxine biosynthetic pathway. Complementation analysis showed that pdxF and pdxC mutations reported previously are most likely alleles of serC. Growth of serC chromosomal insertion mutants on glycoalaldehyde was found to occur without acquisition of second-site mutations and confirmed that pdxB and serC, but not pdxA, fu...Continue Reading

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Citations

Feb 7, 2001·The Journal of Biological Chemistry·M TazoeT Hoshino
Nov 15, 2017·RNA Biology·Etienne B GreenleeRonald R Breaker
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Masaaki TazoeTatsuo Hoshino
Jul 21, 2005·The Journal of Biological Chemistry·Thomas RaschleTeresa B Fitzpatrick
Sep 8, 2010·Molecular Microbiology·Thomas DickMartin Gengenbacher
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Nov 10, 2001·Annual Review of Phytopathology·Margaret E Daub, Marilyn Ehrenshaft
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Oct 14, 2017·Environmental Microbiology·Jonathan RosenbergFabian M Commichau
Sep 29, 2004·The Journal of Biological Chemistry·María B CasseraAlejandro M Katzin
Jan 18, 2007·The Plant Journal : for Cell and Molecular Biology·Olca TitizTeresa B Fitzpatrick
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Mar 6, 2021·Applied Microbiology and Biotechnology·Björn Richts, Fabian M Commichau
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Dec 1, 2004·EcoSal Plus·George V Stauffer
Apr 1, 2021·EcoSal Plus·Angela TramontiRoberto Contestabile

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