Metabolic response to endotoxin in vivo in the conscious mouse: role of interleukin-6.

Metabolism: Clinical and Experimental
Andrea TweedellOwen P McGuinness

Abstract

Inflammation and insulin resistance are characteristics of endotoxemia. Although the role of interleukin (IL)-6 in insulin-resistant states has been characterized, little is known of its role in the metabolic response to inflammation. To study the role of IL-6, conscious chronically catheterized mice were used. Five days before being studied, catheters were implanted in the carotid artery and jugular vein. After a 5-hour fast, Escherichia coli (250 μg per mouse) lipopolysaccharide (LPS) was injected in IL-6⁻/⁻ (KO, n = 13) and IL-6+/+ (WT, n = 10) littermates. The IL-6 response to LPS was simulated in an additional group of KO mice (KO + IL-6, n = 10). Interleukin-6 increased in WT (15 ± 0.7 ng/mL) 4 hours after LPS and was undetectable in KO. Interleukin-6 replacement in the KO restored circulating IL-6 to levels observed in the WT group (14 ± 0.3 ng/mL). Tumor necrosis factor-α increased more rapidly in WT than in both KO and KO + IL-6 mice. The KO mice exhibited a more profound glucose excursion 30 minutes after LPS injection and no apparent hypoglycemia at 4 hours (95 ± 5 vs 70 ± 8 mg/dL, KO vs WT), despite having a blunted glucagon and epinephrine response. Glucose levels in KO + IL-6 mice, while decreased (93 ± 4 mg/dL) a...Continue Reading

References

Nov 30, 1987·Biochemical and Biophysical Research Communications·K MészárosJ J Spitzer
Oct 1, 1994·The Journal of Experimental Medicine·E FattoriV Poli
Sep 5, 1997·The Journal of Biological Chemistry·K D NiswenderM A Magnuson
Jan 23, 1999·The New England Journal of Medicine·A P Wheeler, G R Bernard
Nov 4, 2000·The Journal of Clinical Endocrinology and Metabolism·A O AgwunobiG L Carlson
Apr 1, 1957·The American Journal of Physiology·J S WALLN ALTSZULER
Oct 28, 2003·Diabetes·Peter J KloverRobert A Mooney
Jan 15, 2004·American Journal of Physiology. Endocrinology and Metabolism·Rikke Krogh-MadsenBente Klarlund Pedersen
Jun 12, 2004·American Journal of Physiology. Endocrinology and Metabolism·Gina B Di GregorioPhilip A Kern
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·Shannon CopelandUNKNOWN Inflammation and the Host Response to Injury Investigators
Feb 9, 2006·American Journal of Physiology. Endocrinology and Metabolism·Rikke Krogh-MadsenBente K Pedersen
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Helga EllingsgaardMarc Y Donath
Sep 18, 2008·Diabetes·Iria Nieto-VazquezMargarita Lorenzo

❮ Previous
Next ❯

Citations

Dec 28, 2012·American Journal of Physiology. Endocrinology and Metabolism·Jianping Ye, Owen P McGuinness
Jul 1, 2014·International Journal of Molecular Sciences·René J Hernández-BautistaNorma E López-Diazguerrero
Dec 16, 2016·International Journal of Molecular Sciences·Salvatore PettaFerruccio Bonino
Aug 7, 2014·American Journal of Physiology. Endocrinology and Metabolism·Rebecca E K MacPhersonDavid C Wright
Apr 26, 2020·Developmental Neurobiology·Jie DongIrina Burd
Apr 28, 2016·BMC Pediatrics·Jeanna M HarveyRenee Taylor
Sep 11, 2014·American Journal of Physiology. Endocrinology and Metabolism·Tammy M BarnesOwen P McGuinness
Feb 26, 2015·Physiological Reports·Samyra L BuzelleDavid C Wright
Aug 31, 2021·Infection and Drug Resistance·Xishuai WangYang Gao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Cellular Immunometabolism

Immunometabolism involves not only the way metabolites are digested by the immune system, but the way in which they can alter the pathway of the immune system. These responses occur in day-to-day healthy cell functioning, but can result in diseases, such as autoimmune disorders. Discover the latest research on cellular immunometabolism here.