Metabolic responses of Rhodococcus erythropolis PR4 grown on diesel oil and various hydrocarbons

Applied Microbiology and Biotechnology
Krisztián LacziGábor Rákhely

Abstract

Rhodococcus erythropolis PR4 is able to degrade diesel oil, normal-, iso- and cycloparaffins and aromatic compounds. The complete DNA content of the strain was previously sequenced and numerous oxygenase genes were identified. In order to identify the key elements participating in biodegradation of various hydrocarbons, we performed a comparative whole transcriptome analysis of cells grown on hexadecane, diesel oil and acetate. The transcriptomic data for the most prominent genes were validated by RT-qPCR. The expression of two genes coding for alkane-1-monooxygenase enzymes was highly upregulated in the presence of hydrocarbon substrates. The transcription of eight phylogenetically diverse cytochrome P450 (cyp) genes was upregulated in the presence of diesel oil. The transcript levels of various oxygenase genes were determined in cells grown in an artificial mixture, containing hexadecane, cycloparaffin and aromatic compounds and six cyp genes were induced by this hydrocarbon mixture. Five of them were not upregulated by linear and branched hydrocarbons. The expression of fatty acid synthase I genes was downregulated by hydrocarbon substrates, indicating the utilization of external alkanes for fatty acid synthesis. Moreover, t...Continue Reading

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Citations

Dec 17, 2018·Applied Microbiology and Biotechnology·Jessica ZampolliPatrizia Di Gennaro
Dec 12, 2020·Frontiers in Microbiology·Krisztián LacziKatalin Perei
Oct 31, 2021·Scientific Reports·Jessica ZampolliPatrizia Di Gennaro

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