PMID: 9646503Jul 1, 1998Paper

Metabolic suppression in anoxic frog muscle

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
T G West, R G Boutilier

Abstract

Microcalorimetry is the only direct method for measuring moment-to-moment changes in whole-cell metabolism (as heat output) during anoxia. We have adapted this methodology, in conjunction with standard muscle isolation techniques, to monitor metabolic transitions in isolated frog (Rana temporaria) sartorius muscle during anoxia and recovery (reoxygenation). Anoxia (sustained 1 h, following 2 h progressive hypoxia) suppressed muscle heat output to 20% of the stable normoxic level. This effect was fully reversible upon reoxygenation. Metabolite profiles were consistent with other anoxia-tolerant vertebrates--most notably, adenosine triphosphate (ATP) content during anoxia and reoxygenation remained unchanged from normoxia (pre-anoxic control). In addition, the concentration of K+ ions ([K+]) in interstitial dialysates remained stable (2-3 mM) throughout anoxia and recovery. Interstitial [lactate-] increased slightly, in accord with anaerobiosis supporting suppressed metabolic rates during anoxia. The degree of anoxic suppression of metabolism observed is similar to other vertebrate models of anoxia tolerance. Furthermore, stable ATP concentrations and interstitial [K+] in the isolated tissue suggests that intrinsic mechanisms sup...Continue Reading

Citations

Feb 12, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Cosima PorteusWilliam K Milsom
Oct 26, 2002·Marine Pollution Bulletin·Rudolf S S Wu
Nov 24, 2001·Respiration Physiology·R G Boutilier
Sep 16, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·R G Boutilier, J St-Pierre
Apr 24, 2008·Biological Reviews of the Cambridge Philosophical Society·Glenn J Tattersall, Gordon R Ultsch
Jun 9, 2000·Annual Review of Physiology·A J Hulbert, P L Else
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J St-PierreR G Boutilier
Sep 2, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Leonard G Forgan, Malcolm E Forster
Jun 19, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·James F Staples, Leslie T Buck
Jun 11, 2010·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Donald C Jackson, Gordon R Ultsch
Aug 27, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Cindy D CrockerMery L Martínez
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Jul 12, 2020·The Journal of Experimental Biology·Georgina K Cox, Todd E Gillis
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J St-PierreR G Boutilier
Oct 14, 2003·The Journal of Experimental Biology·Sarah L MiltonPeter L Lutz
Apr 20, 2006·The Journal of Experimental Biology·Silvia Cristina R de Souza, Claudia M Kuribara
Dec 12, 2003·The Journal of Experimental Biology·Silvia Cristina R de SouzaMarilene S C Bianconcini

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