Metabolic transformation of rabbit skeletal muscle cells in primary culture in response to low glucose

Biochimica Et Biophysica Acta
Nina HankeHans-Peter Kubis

Abstract

We have investigated the mechanism of the changes in the profile of metabolic enzyme expression that occur in association with fast-to-slow transformation of rabbit skeletal muscle. The hypotheses assessed are: do 1) lowered intracellular ATP concentration or 2) reduction of the muscular glycogen stores act as triggers of metabolic transformation? We find that 3 days of decreased cytosolic ATP content have no impact on the investigated metabolic markers, whereas incubation of the cells with little or no glucose leads to decreases in glycogen in conjunction with decreases in glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter activity, GAPDH mRNA and specific GAPDH enzyme activity (indicators of the anaerobic glycolytic pathway), and furthermore to increases in mitochondrial acetoacetyl-CoA thiolase (MAT, also known as ACAT) promoter activity, peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) expression and citrate synthase (CS) specific enzyme activity (all indicators of oxidative metabolic pathways). The AMP-activated protein kinase (AMPK) activity under these conditions is reduced compared to controls. In experiments with two inhibitors of glycogen degradation we show that the observed met...Continue Reading

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Citations

Jan 29, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Eric GuévélouCharlotte Corporeau
Jul 16, 2010·European Journal of Applied Physiology·Francesco SartorHans-Peter Kubis
Nov 2, 2014·Journal of Cellular Physiology·Alastair Khodabukus, Keith Baar
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Yoshinobu OhiraNaoya Nakai
Jul 30, 2014·Lab on a Chip·G BergströmN D Robinson

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