PMID: 2114284May 31, 1990Paper

Metabolism and actions of 2-deoxy-2-fluoro-D-galactose in vivo

European Journal of Biochemistry
B R GrünD Keppler

Abstract

The synthetic D-galactose analog 2-deoxy-2-fluoro-D-galactose (dGalF) offers unique advantages for studies of the D-galactose pathway by non-invasive techniques using 19F-NMR spectroscopy or positron emission from the 18F-labeled compound. The metabolism of 2-deoxy-2-fluoro-D-galactose was studied in rodents using the unlabeled, the 18F-labeled, and the 14C-labeled D-galactose analog. Analyses for the metabolites of 2-deoxy-2-fluoro-D-galactose were performed by HPLC, enzymatic methods, and 19F-NMR spectroscopy in vivo and in vitro. The metabolism of 2-deoxy-2-fluoro-D-galactose was most active in the liver which took up the major part of the administered dose of the 14C-labeled D-galactose analog, but renal excretion was also pronounced. This was confirmed by in vivo scanning of the rat using the 18F-labeled sugar (1.5 microCi/g; 25 nmol/g) and examination by positron-emission tomography and gamma camera. The dose dependence of the levels of the hepatic metabolites of 2-deoxy-2-fluoro-D-galactose was investigated for doses between 25 nmol/g body mass and 1 mumols/g body mass. After 1 h, the major part of the acid-soluble uracil nucleotides consisted of UDP-2-deoxy-2-fluoro-D-hexoses when the dose was at least 0.1 mumols/g. Wit...Continue Reading

References

Nov 1, 1977·European Journal of Biochemistry·J J Starling, D O Keppler
Oct 1, 1988·Chemical & Pharmaceutical Bulletin·Y KanazawaT Haradahira
Jul 1, 1987·Journal of Computer Assisted Tomography·S HolteM Kesselberg
Jan 1, 1974·Reviews of Physiology, Biochemistry and Pharmacology·K Decker, D Keppler
Sep 1, 1980·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T AnukarahanontaD O Keppler
May 1, 1968·The Biochemical Journal·F MaleyR Delgiacco

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Citations

Jan 1, 1996·Journal of Surgical Oncology·S RowleyH K Schackert
May 1, 1992·International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes·T HaradahiraK Masuda
May 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael SørensenSusanne Keiding
Feb 11, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Susanne Keiding
Dec 9, 1991·FEBS Letters·N LochW Reutter
Dec 14, 2018·Current Medicinal Chemistry·Sara N GarciaM Matilde Marques

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