Metabolism and actions of ADP-riboses in coronary arterial smooth muscle

Advances in Experimental Medicine and Biology
P LiW B Campbell

Abstract

The present study examined the metabolism of ADP-ribose (ADPR) and cyclic ADP-ribose (cADPR) in small bovine coronary arterial homogenates and characterized the effects of these nucleotides on the activity of potassium (K+) channels in coronary smooth muscle cells. ADPR and cADPR were produced from NAD+ (1mM) by homogenates from small bovine coronary arteries. The conversion rate was 2.81 +/- 0.19 nmol/min/100 micrograms protein for ADPR and 1.37 +/- 0.03 nmol/min/100 micrograms protein for cADPR. In patch clamp experiments, ADPR produced a concentration-dependent increase in the activity of a calcium activated K (Kca) channel in inside-out membrane patches of coronary arterial smooth muscle cells at concentrations of 0.1, 1 and 10 microM. The open state probability (NPo) of Kca channel was maximally increased 5-fold at a concentration of 10 microM. cADPR reduced the activity of Kca channel at concentrations of 1 and 10 microM. The NPo was decreased by 45% and 75%, respectively. The results indicate that there is an enzymatic pathway in the coronary arterial smooth muscle to produce ADPR and cADPR. These nucleotides may play a role in the control of coronary vascular tone by altering the activity of the Kca channel in vascular ...Continue Reading

Citations

Sep 20, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J Z YuP L Li
Feb 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Fan ZhangPin-Lan Li
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·P L LiW B Campbell
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Pin-Lan LiWilliam B Campbell
Oct 26, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Guo ZhangPin-Lan Li
Dec 17, 2004·Pharmacological Reviews·Régent LaporteIsmail Laher
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Wang-Xian TangPin-Lan Li
Jun 27, 2006·Journal of Cellular and Molecular Medicine·Andrew Y Zhang, Pin-Lan Li
Jun 6, 2006·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M ThompsonE N Chini
Jun 22, 1999·Cellular Signalling·A H Guse
Jan 27, 2005·Pharmacology & Therapeutics·Ni BaiIsmail Laher

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