PMID: 9531498Jun 11, 1998Paper

Metabolism and apoptotic properties of elevated ceramide in HT29rev cells

The Biochemical Journal
R J VeldmanJ W Kok

Abstract

Ceramide (Cer) has been implicated in the regulation of apoptosis. In this study, we elevated cellular Cer levels in human colon-carcinoma (HT29(rev)) cells by incubating the cells in the presence of bacterial sphingomyelinase (bSMase) or, alternatively, in the presence of C2-Cer, a short-chain analogue of the sphingolipid. bSMase treatment did not induce apoptosis in these cells, as revealed by a lack of both DNA fragmentation and cleavage of poly(ADP-ribose)polymerase. In contrast, apoptosis did occur upon addition of C2-Cer. These findings led us to study whether differences in the metabolic fate of the excess of Cer, as generated by both treatments, contributed to the observed difference in apoptosis-inducing capacity. C2-Cer was rapidly taken up by HT29(rev) cells and accumulated due to the absence of substantial metabolic conversion. Upon addition of bSMase, hydrolysis of sphingomyelin resulted in a reduction of that pool to 20% compared with control values, accompanied by a multi-fold increase in Cer level. In spite of the continuous presence of active bSMase, the Cer increase turned out to be transient. Cer levels reached their maximum 1-2 h after addition of bSMase, followed by a significant decrease. Excessive Cer was...Continue Reading

Citations

Mar 24, 1999·Molecular Membrane Biology·D J Sillence, D Allan
Sep 26, 2013·Biochimica Et Biophysica Acta·Mónica García-BarrosYusuf A Hannun
Sep 19, 2002·International Journal of Cancer. Journal International Du Cancer·Claire Rodriguez-LafrasseRobert Rousson
Jun 1, 2015·Biochemical and Biophysical Research Communications·Weihong XuJie Shen
Mar 1, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Long ChengXu-Ming Bai
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Aug 30, 2011·Reproduction, Fertility, and Development·Dorit Kalo, Zvi Roth
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robert Jan VeldmanJan Willem Kok
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Jul 2, 1998·Biochemical and Biophysical Research Communications·R J VeldmanJ W Kok
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Jan 18, 2003·Biochimica Et Biophysica Acta·Benjamin J PettusYusuf A Hannun

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