PMID: 8600984Mar 15, 1996Paper

Metabolism and detoxification of nitrite by trout hepatocytes

Biochimica Et Biophysica Acta
C Doblander, R Lackner

Abstract

Nitrite (NO2-) is one of the most important toxicants to fish. Freshwater fish are especially sensitive, particularly salmonids. Nitrite uptake is thought to occur via the HCO3-, Cl- -exchanger at the gill epithelia with nitrite substituting for chloride. In this way freshwater fish accumulate nitrite in the blood up to 100-fold from the surrounding water. Another source, endogenous nitrite as a degradation product of nitric oxide, rarely leads to pharmacologically relevant concentrations. We developed a new method for the determination of nitrate (NO3-) in biological samples and used it to measure nitrite oxidation in isolated rainbow trout (Oncorhynchus mykiss) hepatocytes which were found to detoxify nitrite to the almost non-toxic nitrate. Detoxification is inhibited by 0.05 mM bumetanide and 0.1 mM furosemide but not by SITS and DITS, suggesting the involvement of the Na+, K+, 2Cl- -cotransporter with nitrite or nitrate substituting for chloride. Oxidation of nitrite is strongly accelerated by 0.05 mM uric acid. The efficacy of this antioxidant suggests that similar reactions are involved as known for haemoglobin [33]. However, in the case of trout liver also membrane bound detoxificating activity can be observed which is ...Continue Reading

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Citations

Apr 6, 2004·Aquatic Toxicology·Onkar S Bains, Christopher J Kennedy
Aug 10, 2000·Aquatic Toxicology·M Grosell, F B Jensen
Aug 6, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cláudia B R Martinez, Marta M Souza
May 3, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Frank B Jensen
Jun 19, 2010·European Journal of Histochemistry : EJH·I N VatsosM Alexis
Jan 11, 2011·Aquatic Toxicology·Frank B Jensen, Marie N Hansen
Oct 28, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Meiling HongNa Yu
Nov 24, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Ive M AvilezGilberto Moraes
Dec 3, 2014·Water Research·Podmirseg Sabine MarieBernhard Wett
Nov 5, 2019·Frontiers in Microbiology·Pamela VrablWolfgang Burgstaller
Mar 1, 1997·Applied and Environmental Microbiology·D De BeerM Kuhl

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