Metabolism and pharmacokinetics of orally administered saikosaponin b1 in conventional, germ-free and Eubacterium sp. A-44-infected gnotobiote rats

Biological & Pharmaceutical Bulletin
H KidaM Hattori

Abstract

The metabolic fate of saikosaponin b1 (1) was investigated using conventional, germ-free and Eubacterium sp. A-44-infected gnotobiote rats. After the oral administration of 1 to germ-free rats at a dose of 50 mg/kg, no metabolite was detected in the plasma, the cecal contents or the cumulative feces through the experiment. On the other hand, when 1 was orally given to the Eubacterium sp. A-44-infected gnotobiote rats, considerable amounts of its metabolites, prosaikogenin A (2) and saikogenin A (3), were detected in the rat plasma with the respective AUC0-10 h values of 17,424 and 22,260 pmol.min/ml, similar to the case of its oral administration to conventional rats (AUC0-10 h values of 9,936 and 12,414 pmol.min/ml for 2 and 3, respectively). Furthermore, significant amounts of both metabolites were detected in the cecal contents and the cumulative feces of the gnotobiote and conventional rats, but not in those of the germ-free rats, within 10 h after the administration. Fecal and cecal activities of hydrolyzing 1 and 2 were found in the gnotobiote and conventional rats, though there were no detectable activities in the germ-free rats. Accordingly, both hydrolyzing activities in the intestinal bacteria, such as Eubacterium sp....Continue Reading

Citations

Nov 6, 2010·Obstetrics and Gynecology International·J L Fernández-MoreraM F Fraga
Apr 8, 2016·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Pei YuRui Song
Mar 20, 2014·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Xianzhi ChenShirui Mao
Feb 2, 2010·Biological & Pharmaceutical Bulletin·Ge XuChanglin Zhou
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi HeShuiyuan Cheng
Jan 2, 2013·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Guoqiang LiuZunjian Zhang
Dec 21, 2019·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Cui TangYing Guo
Oct 19, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ting DuGuangde Yang

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