PMID: 1204615Nov 1, 1975Paper

Metabolism of [4-14C] testosterone in the rat uterus in vitro

European Journal of Biochemistry
U HoffmannB P Lisboa

Abstract

In view of the uterine action of androgens we have investigated in vitro the metabolism of [4-14C]-testosterone in uterine tissue of ovariectomized rats. After purification of the extracts on Amberlite XAD-2 the metabolites have been isolated by gel. Five metabolites were isolated and identified during these incubation studies: 4-androstene 3,17-dione, 17beta-hydroxy-5alpha-androstan-3-one, 5 alpha-androstane-3alpha17beta-diol, 4-androstene-3 beta, 17beta-diol and 4-androstene-3alpha, 17beta-diol. Furthermore, two polar C19O3-metabolites and one isopolar to 5 alpha-androstane-3, 17-dione have also been detected. The metabolites were characterized by radioactive gas chromatogrphy, and determination of the relative specific activity in the eluates of Sephadex column chromatography. The identification of allylic alcohols was complemented by their oxidation to 4-androstene-3,17-dione. The present data show that activity of 17beta,3alpha- and 3beta-hydroxysteroid-oxidoreductase and 5alpha-ring-reductase are involved in the metabolism of testosterone in vitro in the rat uterus. The very low 5 alpha-reductase activity under the experimental conditions used in this work explains the formation of allylalcohols as the principal metabolit...Continue Reading

References

Aug 20, 1975·Journal of Chromatography·B P Lisboa, M Strassner
Jul 1, 1975·Journal of Steroid Biochemistry·B P Lisboa, H D Sauer
Jul 1, 1975·Journal of Steroid Biochemistry·B P Lisboa, H D Sauer
Aug 20, 1971·Biochemical and Biophysical Research Communications·G Giannopoulos
Sep 1, 1966·The Journal of Clinical Endocrinology and Metabolism·H L BradlowT F Gallagher
Jun 1, 1966·Biochemistry·D K FukushimaT F Gallagher
Jun 1, 1963·Analytical Biochemistry·M MAKITA, W W WELLS
Mar 1, 1963·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H BORGSTEDEJ ZANDER

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Citations

Nov 1, 1976·Journal of Steroid Biochemistry·W HeynsP de Moor
Jul 1, 1977·Steroids·H JunkermannB P Lisboa

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