PMID: 7082654Apr 13, 1982Paper

Metabolism of 5,6-epoxyretinoic acid in vivo: isolation of a major intestinal metabolite

Biochemistry
Joseph L NapoliA M McCormick

Abstract

The major metabolite in the small intestinal mucosa of vitamin A deficient rats dosed intrajugularly with 5,6-epoxy[3H]-retinoic acid has been identified as 5,6-epoxyretinoyl beta-glucuronide. The assignment was based on the metabolite's chemical, spectral, and chromatographic properties. Incubation of the metabolite with beta-glucuronidase released 5,6-epoxyretinoic acid. Incubation of 5,6-epoxyretinoic acid with rat liver microsomes in the presence of uridine-5'-diphospho-1 alpha-D-glucuronic acid produced the metabolite. 5,6-Epoxy[3H]retinoyl beta-glucuronide weas observed in the liver, small intestinal mucosa, and intestinal contents but not in kidney of vitamin a deficient rats. Its concentration was greatly diminished in liver and small intestinal mucosa, and it was not observed in kidney of vitamin A deficient rats dosed orally with retinoic acid for several days before administration of 5,6-epoxy[3H]retinoic acid. Generally, oral retinoic acid treatment accelerated 5,6-epoxyretinoic acid metabolism and enhanced accumulation of highly polar metabolites. Moreover, 5,6-epoxyretinoic acid metabolism was more rapid than that of retinoic acid and did not result in production of retinoic acid.

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Joseph L NapoliH F DeLuca
Sep 9, 1976·Nature·M B SpornW R Henderson
Apr 1, 1970·Lipids·R L Geison, B C Johnson
Jul 27, 1966·Biochimica Et Biophysica Acta·P E DUNAGINJ A OLSON
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M H ZileH F DeLuca
Oct 23, 1981·Biochimica Et Biophysica Acta·Joseph L Napoli, A M McCormick
Jan 1, 1980·Journal of Biomedical Engineering·S A Jackson
Feb 15, 1980·The Biochemical Journal·A M McCormickH F DeLuca
May 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·J E Dowling, G Wald
Dec 1, 1966·The Biochemical Journal·B Morgan, J N Thompson

Citations

Jul 16, 2002·Biochimica Et Biophysica Acta·Carsten K SchmidtHeinz Nau
Oct 16, 1999·Biochimica Et Biophysica Acta·Joseph L Napoli
Mar 24, 2007·Biochemistry·Alexander R MoiseWilliam S Blaner
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J B Williams, Joseph L Napoli
Oct 12, 1990·Journal of Chromatography·R Wyss
Jan 1, 1991·Drug Metabolism Reviews·D L Hill, B P Sani
Oct 1, 1986·Journal of the American Academy of Dermatology·F Chytil
Dec 1, 1988·Toxicology and Applied Pharmacology·D M KochharM A Satre
May 31, 2011·Biochimica Et Biophysica Acta·Joseph L Napoli

Related Concepts

5,6-epoxyretinoic acid, sodium salt
5,6-epoxyretinoyl beta-glucuronide
High Pressure Liquid Chromatography Procedure
Structure of Intestinal Gland
Intestines, Small
Microsomes, Liver
August Rats
Vesanoid
Vitamin A Deficiency

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.