PMID: 6538799May 11, 1984Paper

Metabolism of 5(6)-expoxyeicosatrienoic acid by ram seminal vesicles. Formation of novel prostaglandin E1 metabolites

Biochimica Et Biophysica Acta
E H Oliw

Abstract

5(6)-Epoxy-8,11,14-eicosatrienoic acid was incubated with microsomes or ram seminal vesicles in the presence of glutathione (1 mM) for 2 min at 37 degrees C. Following extractive isolation on octadecasilane silica, the products were purified on straight-phase HPLC and separated into three major polar metabolites, which all showed maximal ultraviolet absorbance at 278 nm after treatment with alkali. The least-polar of the three metabolites was identified by capillary column gas chromatography-mass spectrometry as 5(6)- epoxyprostaglandin E1 and the structure was confirmed by comparison with authentic material. The most-polar metabolite was identified as 5,6- dihydroxyprostaglandin E1, while the metabolite of medium polarity was identified as its delta 5-lactone. When glutathione was omitted, 5-hydroxyprostaglandin I 1 alpha and 5-hydroxyprostaglandin I 1 beta were previously identified as the two major metabolites. These results indicate that the postulated epoxyprostaglandin endoperoxide intermediates, 5(6)- epoxyprostaglandin G1 and 5(6)- epoxyprostaglandin H1, might be substrates for the endoperoxide E isomerase enzyme, since this enzyme requires glutathione as a cofactor.

References

Mar 1, 1978·Prostaglandins·J C SihD R Graber
Jul 29, 1983·Biochemical and Biophysical Research Communications·J R FalckJ Capdevila
Feb 11, 1982·Biochemical and Biophysical Research Communications·N ChacosJ Capdevila
Jun 1, 1983·Archives of Biochemistry and Biophysics·N ChacosR W Estabrook

❮ Previous
Next ❯

Citations

Nov 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·H C Hercule, A O Oyekan
Feb 3, 2007·The Journal of Pharmacology and Experimental Therapeutics·K Trent MorelandAlan H Stephenson
May 1, 1986·Acta Physiologica Scandinavica·E H OliwM Hamberg
May 14, 2014·Annual Review of Nutrition·David Bishop-BaileyCaroline Wheeler-Jones
Aug 5, 1998·The American Journal of Physiology·A H StephensonA J Lonigro
Aug 30, 2000·Prostaglandins & Other Lipid Mediators·J H Capdevila, J R Falck
Nov 27, 1999·Molecular Aspects of Medicine·J A Hasler
Feb 28, 1992·Biochemical and Biophysical Research Communications·J Y ZhangI A Blair
Feb 15, 1985·Biochemical and Biophysical Research Communications·E H Oliw, G Benthin
Nov 7, 2009·Journal of Molecular and Cellular Cardiology·Yangmei DengCraig R Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.