PMID: 3768058Oct 1, 1986Paper

Metabolism of a cartilage matrix glycoprotein in normal and osteoarthritic canine articular cartilage

Arthritis and Rheumatism
R S FifeK D Brandt

Abstract

We have recently described a 550,000-dalton noncollagenous cartilage matrix glycoprotein (CMGP), with subunits of 130,000, which is present in hyaline cartilage and fibrocartilage. Biosynthetic studies indicated that CMGP was synthesized by short-term organ cultures of normal canine articular cartilage, representing approximately 9% of the total 3H-leucine incorporated into protein in 24-hour cultures. There was no incorporation of 35S-sulfate or 3H-mannose into CMGP under these conditions, but the protein did incorporate 32P-phosphate. The majority of the 3H-leucine-labeled CMGP was removed after 24 hours of chase with unlabeled leucine, and only a small amount remained at 72 hours, which suggests that there was rapid metabolism of the protein. CMGP was not detected in cartilage after addition of cycloheximide to the culture medium; this confirms its short half-life. Cultures of osteoarthritic cartilage obtained from dogs 8-10 weeks after anterior cruciate ligament transection revealed no difference in the metabolism of CMGP in this tissue compared with that found in cultures of normal articular cartilage.

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Citations

Sep 1, 1992·Arthritis and Rheumatism·P Witsch-PrehmH Kresse
Jan 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P DasR L Smith
Aug 1, 1994·Calcified Tissue International·R S FifeL M Ryan
Sep 28, 1994·Biochimica Et Biophysica Acta·R S FifeC Proctor
May 7, 1998·Cancer Letters·R S FifeC Proctor
Jun 10, 1998·Cancer Letters·R S FifeC Proctor
Jan 1, 1997·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·X Chevalier
Mar 22, 2003·Journal of Structural Biology·I ZizakP Fratzl
Nov 1, 1989·The Journal of Clinical Investigation·R S Fife, K D Brandt
Aug 1, 1989·Annals of the Rheumatic Diseases·M Doherty
May 20, 2020·Frontiers in Bioengineering and Biotechnology·Weiping LinGang Li

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