Metabolism of angiotensins by head membranes of the leech Theromyzon tessulatum

FEBS Letters
V Laurent, M Salzet

Abstract

Angiotensins (angiotensin I, angiotensin II, angiotensin II-amide) have been isolated in leeches and such peptides are involved in diuresis in these animals. To explore possible inactivation mechanisms of these peptides, angiotensins were incubated with head membranes of the leech T. tessulatum. Membranes derived from head parts of this leech are very rich in peptidases. They contain endopeptidase-24.11-like enzyme (NEP-like) associated with a battery of exopeptidase. The way that angiotensins are degraded by the combined attack of these membrane peptidases has been investigated. The contribution of individual peptidases was assessed by adding inhibitors (phosphoramidon, captopril and amastatin) to the membrane fractions, when they were incubated with the peptides. In the case of angiotensin I, the primary attack was performed by a combined action of the NEP-like and the ACE-like enzymes, followed by aminopeptidase attacks. Angiotensin II and III were hydrolyzed by NEP-like enzyme at the same Tyr-Ile bond, whereas the N-terminal arginine residue of angiotensin III was removed by an arginyl aminopeptidase. These results show that angiotensins are efficiently degraded by membranes and that NEP-like enzyme plays a key role in this...Continue Reading

References

Dec 1, 1976·The Journal of Clinical Endocrinology and Metabolism·T T GuyeneP Corvol
Jan 1, 1992·Comparative Biochemistry and Physiology. A, Comparative Physiology·M SalzetJ Malecha
Oct 1, 1995·European Journal of Biochemistry·V Laurent, M Salzet
Sep 23, 1994·Journal of Chromatography. B, Biomedical Applications·G W WellingS Welling-Wester

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Citations

Aug 27, 1998·Immunology Letters·G B StefanoM Salzet
Oct 13, 2001·Brain Research. Molecular Brain Research·M Salzet, M Verger-Bocquet
Jan 8, 1999·Brain Research. Molecular Brain Research·G B StefanoM Salzet
Mar 17, 2000·Brain Research. Molecular Brain Research·M Salzet, L Deloffre
Jul 4, 1997·Molecular and Cellular Endocrinology·M Salzet, G Stefano
Feb 4, 2010·Journal de la Société de biologie·Guillaume Rivière
May 16, 2008·Archives of Insect Biochemistry and Physiology·J Sook Chung, S G Webster

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