Metabolism of arachidonic acid in vitro by porcine blastocysts and endometrium

Prostaglandins
G S Lewis, R A Waterman

Abstract

Metabolism of radiolabeled arachidonic acid (*AA) by blastocysts and endometrial slices recovered from five gilts 16 days after detection of estrus was studied in vitro. Blastocysts from each gilt were divided into four 216 +/- 18 mg portions, and each portion was placed into a separate petri dish containing 15 ml modified minimum essential medium (MEM). The incubates from each gilt received either 25, 50, 100 or 200 micrograms radioinert arachidonic acid (AA). Endometrium was dissected from each uterine horn, sliced and duplicate 509 +/- 3 mg portions from each gilt were placed into petri dishes containing 15 ml MEM and 200 micrograms AA. All incubates received 5 microCi of *AA (either [14C]-arachidonic acid or [3H]-arachidonic acid). The incubates were rocked at 37 degrees C for 24 h in an atmosphere of 50% N2:45% O2:5% CO2. After incubation, tissues and MEM were separated by centrifugation. Metabolism of *AA was assessed in extracts of MEM and tissue homogenates by separating *AA and its metabolites on columns of Sephadex LH-20. Blastocysts produced compounds that migrated with [3H]-13,14-dihydro-15-keto-PGF2 alpha (*PGFM), [3H]-PGE2 (*PGE2) and [3H]-PGF2 alpha (*PGF2 alpha). The greatest (P less than .05) proportion (35.7 +...Continue Reading

References

Oct 1, 1978·Biology of Reproduction·J D BiggersJ Fried
Jul 1, 1977·Journal of Reproduction and Fertility·L H HoffmanJ C Frölich
May 1, 1972·Developmental Biology·L G Barth, L J Barth
Sep 1, 1982·Biology of Reproduction·G S LewisJ S Curl
Mar 1, 1980·Prostaglandins·S K DeyR D Crist

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Citations

Dec 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R W Silcox, B H Johnson
Mar 1, 1990·Journal of Reproductive Immunology·P V HolmesL Hamberger
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·T Yoshimoto, S Yamamoto
Apr 4, 1998·European Journal of Pharmacology·Z MezeiS Sonkodi
Jun 6, 2002·Animal Reproduction Science·Matthew E WilsonStephen P Ford
Jun 30, 2000·Seminars in Cell & Developmental Biology·J A Bowen, R C Burghardt
Apr 30, 1999·Physiological Reviews·J A McCrackenJ C Lamsa

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