Metabolism of H2-CO2, methanol, and glucose by Butyribacterium methylotrophicum.

Journal of Bacteriology
L Lynd, J G Zeikus

Abstract

The fermentative metabolism of Butyribacterium methylotrophicum grown on either H2-CO2, methanol, glucose, or CO is described. The following reaction stoichiometries were obtained: 1.00 H2 + 0.52 CO2 leads to 0.22 acetate + 0.06 cell C; 1 methanol + 0.18 CO2 + 0.01 acetate leads to 0.24 butyrate + 0.29 cell C; and 1.00 glucose leads to 0.31 CO2 + 1.59 acetate + 0.21 butyrate + 0.13 H2 + 1.58 cell C. Cell yields of 1.7 g (dry weight) per mol of H2, 8.2 g (dry weight) per mol of methanol, 42.7 g (dry weight) per mol of glucose, and 3.0 g (dry weight) per mol of CO were obtained from linear plots of cell synthesis and substrate consumption. Doubling times of 9.0, 9.0, and 3 to 4 h were observed during batch growth on H2-CO2, methanol, and glucose, respectively. Indicative of a growth factor limitation, glucose fermentation in defined medium displayed a lower cell synthesis efficiency than when yeast extract (0.05%) was present. B. methylotrophicum fermentation displayed atypically high substrate/cell carbon synthesis conversion ratios for an anaerobe, as greater than 24% of the carbon was assimilated into cells during growth on methanol or glucose. The data indicate that B. methylotrophicum conserves carbon-bound electrons during ...Continue Reading

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Citations

Oct 1, 1991·Antonie van Leeuwenhoek·J J Heijnen
Dec 3, 2016·Genome Announcements·Frank R BengelsdorfPeter Dürre
Dec 20, 2013·Environmental Technology·Sean NguyenSonia M Tiquia-Arashiro
Dec 5, 2015·Frontiers in Microbiology·Martijn DienderDiana Z Sousa
Apr 5, 1992·Biotechnology and Bioengineering·J J Heijnen, J P Van Dijken
Sep 20, 2020·Applied and Environmental Microbiology·Ye YaoHe Liu
Sep 10, 2020·FEMS Microbiology Reviews·Florian Kremp, Volker Müller
Jul 14, 2018·Environmental Microbiology·Florian KrempVolker Müller
May 1, 1984·Applied and Environmental Microbiology·W H Lorowitz, M P Bryant
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May 20, 2021·Journal of Applied Microbiology·Xiangan Li, Michael A Henson
Mar 8, 1985·Science·J G ZeikusJ A Krzycki

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