Metabolism of lactose by Clostridium thermolacticum growing in continuous culture

Archives of Microbiology
Christophe ColletP Soucaille

Abstract

The objective of the present study was to characterize the metabolism of Clostridium thermolacticum, a thermophilic anaerobic bacterium, growing continuously on lactose (10 g l(-1)) and to determine the enzymes involved in the pathways leading to the formation of the fermentation products. Biomass and metabolites concentration were measured at steady-state for different dilution rates, from 0.013 to 0.19 h(-1). Acetate, ethanol, hydrogen and carbon dioxide were produced at all dilution rates, whereas lactate was detected only for dilution rates below 0.06 h(-1). The presence of several key enzymes involved in lactose metabolism, including beta-galactosidase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate:ferredoxin oxidoreductase, acetate kinase, ethanol dehydrogenase and lactate dehydrogenase, was demonstrated. Finally, the intracellular level of NADH, NAD+, ATP and ADP was also measured for different dilution rates. The production of ethanol and lactate appeared to be linked with the re-oxidation of NADH produced during glycolysis, whereas hydrogen produced should come from reduced ferredoxin generated during pyruvate decarboxylation. To produce more hydrogen or more acetate from lactose, it thus appears that an efficient...Continue Reading

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Citations

Aug 29, 2012·Microbial Cell Factories·Simon Rittmann, Christoph Herwig
Sep 22, 2012·Applied Microbiology and Biotechnology·Stefan de KokRobbert Kleerebezem
Feb 4, 2014·Bioprocess and Biosystems Engineering·Lei Xu, Ulrike Tschirner
Jun 23, 2009·Biotechnology Advances·R GheshlaghiC P Chou
Jul 24, 2008·Metabolic Engineering·Chung-Jung ChouRobert M Kelly

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