PMID: 6161005Nov 1, 1980Paper

Metabolism of lipid-linked oligosaccharide intermediates in rat spleen lymphocytes. Evidence for ectoglycosyltransferase activities

European Journal of Biochemistry
B HoflackA Verbert

Abstract

Double-labelling experiments show that intact lymphocytes as well as lymphocyte homogenates can utilize GDP-[14C]mannose and UDP-N-[3H]acetylglucosamine to synthesize lipid-linked oligosaccharide intermediates. However, the intermediates formed are quantitatively and qualitatively different in the two systems. The amount of dolichyl diphosphate oligosaccharides synthesized in both cases was calculated by using external labelling by sodium boro[3H]hydride reduction of the glycan moiety obtained after mild acid treatment of [14C]mannose-labelled dolichyl diphosphate oligosaccharides. This showed that, due to the liberation of intracellular enzymes, a larger amount of dolichyl diphosphate oligosaccharides was synthesized by homogenate. However, this higher glycosyltransferase activity was not detected by the direct measurement of incorporation of labelled GDP-[14C]mannose and UDP-N-[3H]acetylglucosamine, due to isotopic dilution caused by both endogenous soluble UDP-N-acetylglucosamine and membrane-bound dolichyl phosphate mannose accumulated during the homogenization process. In addition, endogenous UDP-glucose allowed the formation, by homogenate, of glucosylated dolichyl diphosphate oligosaccharides which were not observed with...Continue Reading

References

Jul 17, 1978·European Journal of Biochemistry·B HoflackA Verbert
Jan 1, 1978·Journal of Supramolecular Structure·W Deppert, G Walter
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S J TurcoP W Robbins
Jan 1, 1976·Annual Review of Biochemistry·C J Waechter, W J Lennarz
Mar 1, 1977·FEBS Letters·A VerbertJ Montreuil
Aug 24, 1976·Biochimica Et Biophysica Acta·L M Patt, W J Grimes
Jan 1, 1976·Biochimie·G StreckerJ P Farriaux
Nov 1, 1976·European Journal of Biochemistry·A VerbertJ Montreuil
Mar 15, 1976·FEBS Letters·R CacanJ Montreuil
Dec 29, 1975·Biochimica Et Biophysica Acta·B D Shur, S Roth
Feb 1, 1973·The Journal of Cell Biology·J W DePierre, M L Karnovsky
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·N H Behrens, L F Leloir
May 1, 1980·European Journal of Biochemistry·R CacanA Verbert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.