Metabolism of native and naturally occurring multiple modified low density lipoprotein in smooth muscle cells of human aortic intima

Experimental and Molecular Pathology
V V Tertov, A N Orekhov

Abstract

The subfraction of low density lipoprotein (LDL) with low sialic acid content that caused accumulation of cholesterol esters in human aortic smooth muscle cells has been found in the blood of coronary atherosclerosis patients. It was demonstrated that this subfraction consists of LDL with small size, high electronegative charge, reduced lipid content, altered tertiary structure of apolipoprotein B, etc. LDL of this subfraction is naturally occurring multiple-modified LDL (nomLDL). In this study we compared the binding, uptake and proteolytic degradation of native LDL and nomLDL by smooth muscle cells cultured from human grossly normal intima, fatty streaks, and atherosclerotic plaques. Uptake of nomLDL by normal and atherosclerotic cells was 3.5- and 6-fold, respectively, higher than uptake of native LDL. Increased uptake of nomLDL was due to increased binding of this LDL by intimal smooth muscle cells. The enhanced binding is explained by the interaction of nomLDL with cellular receptors other than LDL-receptor. Modified LDL interacted with the scavenger receptor, asialoglycoprotein receptor, and also with cell surface proteoglycans. Rates of degradation of nomLDL were 1.5- and 5-fold lower than degradation of native LDL by no...Continue Reading

References

Apr 1, 1977·European Journal of Biochemistry·H KirschkeP Bohley
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Jun 22, 1992·Biochimica Et Biophysica Acta·W JessupR T Dean
Feb 1, 1992·The Journal of Clinical Investigation·U SaxenaI J Goldberg
Dec 1, 1972·The Journal of Antibiotics·H IkezawaH Umezawa
Jan 1, 1983·Methods in Enzymology·J L GoldsteinM S Brown
Jul 1, 1980·Circulation Research·S WallensteinJ L Fleiss
Nov 1, 1980·European Journal of Biochemistry·D R van der WesthuyzenG A Coetzee

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Citations

Jan 30, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Denise MafraMaurilo Leite
Apr 21, 2006·Clinical Biochemistry·Karina P Gopaul, Martin A Crook
Aug 8, 2014·Oxidative Medicine and Cellular Longevity·Alexander N OrekhovIgor A Sobenin
Sep 9, 2015·Oxidative Medicine and Cellular Longevity·Alexander N OrekhovYuri V Bobryshev
Mar 17, 2007·Bulletin of Experimental Biology and Medicine·N A NikitinaA N Orekhov
Jan 25, 2005·Bulletin of Experimental Biology and Medicine·O M PanasenkoA N Orekhov
Nov 21, 2007·Bulletin of Experimental Biology and Medicine·D V AksenovA N Orekhov
Oct 30, 2004·Bulletin of Experimental Biology and Medicine·I V SuprunA N Orekhov
Jan 22, 2019·Current Opinion in Lipidology·Collin S PrymaGordon A Francis
May 25, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Suzanne P M LutgensSylvia Heeneman
Jul 25, 2019·International Journal of Molecular Sciences·Volha I SummerhillAlexander N Orekhov
Nov 9, 2000·American Journal of Clinical Pathology·M H KrollI Jialal
Sep 16, 2019·Lipids in Health and Disease·Chao ZhangDanyan Xu
Nov 20, 2018·International Journal of Rheumatic Diseases·Marina RodríguezAntoni Castro
Jan 10, 2019·Current Drug Discovery Technologies·Alexander N OrekhovDimitry A Chistiakov
Sep 5, 2020·Cardiovascular Research·Angela PirilloGiuseppe Danilo Norata

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