PMID: 6160586Oct 1, 1980Paper

Metabolism of native and of lactosylated human low density lipoprotein: evidence for two pathways for catabolism of exogenous proteins in rat hepatocytes

Proceedings of the National Academy of Sciences of the United States of America
A D AttieD Steinberg

Abstract

Human low density lipoprotein (LDL) covalently conjugated with 200-250 residues of lactose per LDL particle (Lac-LDL) was bound and rapidly taken up by the galactose-specific receptor of rat hepatocytes. Uptake of Lac-LDL was associated with inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase and stimulation of cholesterol esterification. Uptake of native human LDL had no significant effects on these enzyme activities even when the rates of LDL uptake equaled those of Lac-LDL. When injected into rats, Lac-LDL was selectively removed by the liver (98% of injected dose). The hepatic subcellular distribution of simultaneously injected native 125I-labeled LDL and 131I-labeled Lac-LDL differed significantly, Lac-LDL was associated with fractions enriched in lysosomal hydrolases whereas native LDL was found predominantly in the supernatant fraction enriched in lactate dehydrogenase. Chloroquine (0.1 mM) markedly suppressed uptake of Lac-LDL by cultured rat hepatocytes (> 80%) but had only a small effect on uptake of native LDL. Leupeptin (0.625 mM) inhibited degradation of Lac-LDL more than it did degradation of native LDL. Colchicine (0.25 microM) dramatically suppressed uptake of Lac-LDL (> 70%) but did not affect native LDL upt...Continue Reading

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Citations

Jul 26, 1984·Biochimica Et Biophysica Acta·N DashtiP Alaupovic
Jan 1, 1991·The International Journal of Biochemistry·M J GeelenC H Paays
Jun 1, 1992·Antiviral Research·D K MeijerG Molema
Aug 1, 1981·Lancet·G Gregoriadis
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J C RobbinsT Y Shen
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D B CawleyH R Herschman
Feb 23, 2000·The Journal of Clinical Investigation·J TwiskA D Attie
Jul 18, 1986·Biochimica Et Biophysica Acta·J E TriauE J Schaefer
Dec 1, 1984·Experimental Cell Research·S T MosleyR G Anderson
Jan 1, 1984·CRC Critical Reviews in Biochemistry·A L Schwartz
Sep 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J Stockert, A G Morell
Mar 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A D AttieD Steinberg
Jun 20, 2018·Cancer Chemotherapy and Pharmacology·Mohammad MahmoudianAhmad Yari Khosroushahi
May 25, 2018·Nucleic Acid Therapeutics·Aaron D Springer, Steven F Dowdy
Jan 1, 1983·The American Journal of Physiology·S YedgarD Steinberg

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