Metabolism of PER.C6 cells cultivated under fed-batch conditions at low glucose and glutamine levels

Biotechnology and Bioengineering
Luis Maranga, Charles F Goochee

Abstract

This is the first study to examine PER.C6 cell glucose/energy and glutamine metabolism with fed-batch cultures at controlled low glutamine, low glucose, and simultaneous low glucose and low glutamine levels. PER.C6(TM) cell metabolism was investigated in serum-free suspension bioreactors at two-liter scale. Control of glucose and/or glutamine concentrations had a significant effect on cellular metabolism leading to an increased efficiency of nutrient utilization, altered byproduct synthesis, while having no effect on cell growth rate. Cultivating cells at a controlled glutamine concentration of 0.25 mM reduced q(Gln) and q(NH(4)(+)) by approximately 30%, q(Ala) 85%, and q(NEAA) 50%. The fed-batch control of glutamine also reduced the overall accumulation of ammonium ion by approximately 50% by minimizing the spontaneous chemical degradation of glutamine. No major impact upon glucose/energy metabolism was observed. Cultivating cells at a glucose concentration of 0.5 mM reduced q(Glc) about 50% and eliminated lactate accumulation. Cells exhibited a fully oxidative metabolism with Y(O(2)/Glc) of approximately 6 mol/mol. However, despite no increase in q(Gln), an increased ammonium ion accumulation and Y(NH(4)(+)/Gln) were also obs...Continue Reading

References

Jan 1, 1994·Biotechnology Progress·T A BibilaJ G Aunins
Jan 1, 1995·Biotechnology Progress·T A Bibila, D K Robinson
Apr 15, 1993·The Biochemical Journal·J C StreetK M Brindle
May 15, 1996·Annals of the New York Academy of Sciences·L HäggströmL Ohman
May 15, 1996·Journal of Biotechnology·M SchneiderU von Stockar
Mar 6, 1998·Journal of Biotechnology·M DoverskogL Häggström
Jul 3, 1999·Applied Microbiology and Biotechnology·H J CruzM J Carrondo
Jul 9, 1999·Biotechnology and Bioengineering·A Christie, M Butler
Aug 12, 1999·Biotechnology Progress·P SiegwartA Kamen
Nov 24, 1999·Biotechnology and Bioengineering·H J CruzM J Carrondo
Jun 23, 2000·Enzyme and Microbial Technology·H J CruzM J Carrondo
Apr 5, 2001·Current Opinion in Biotechnology·L Chu, D K Robinson
Feb 8, 2003·Biotechnology Progress·David JonesAbraham Bout
Oct 20, 2005·Journal of Bioscience and Bioengineering·A GambhirW S Hu
Mar 5, 1994·Biotechnology and Bioengineering·M NewlandL K Nielsen
Jun 20, 1994·Biotechnology and Bioengineering·T RyllR Wagner
Sep 20, 1994·Biotechnology and Bioengineering·J Ljunggren, L Häggström
Jul 19, 2008·Biotechnology and Bioengineering·W ZhouJ Aunins

❮ Previous
Next ❯

Citations

Aug 1, 2007·Applied Microbiology and Biotechnology·Marco OldigesChristiane Wiendahl
Jul 25, 2013·Biotechnology Letters·Yeon Jung KimGyun Min Lee
Nov 20, 2013·Journal of Biotechnology·Cédric ValléeOlivier Henry
Feb 16, 2012·The Journal of Biological Chemistry·Ana S AlmeidaHelena L A Vieira
Dec 24, 2013·Applied Microbiology and Biotechnology·Judith WahrheitElmar Heinzle
Apr 3, 2014·Biotechnology Progress·Zahra SheikholeslamiOlivier Henry
May 23, 2015·Applied Microbiology and Biotechnology·R CastroA S Coroadinha
Jun 11, 2015·Human Vaccines & Immunotherapeutics·Xu-Ping LiuZe Chen
May 17, 2015·Protein Expression and Purification·Huihui LiuWenling Han
Aug 10, 2011·Journal of Biotechnology·Alexander StrigunElmar Heinzle
Dec 13, 2016·Journal of Biotechnology·Eric KarengeraOlivier Henry
Feb 1, 2018·International Journal of Molecular Sciences·Nathaniel W Freund, Matthew S Croughan
Dec 30, 2014·Biotechnology and Bioengineering·Tharmala TharmalingamChetan T Goudar
Dec 3, 2008·Biotechnology and Bioengineering·Suresh SelvarasuDong-Yup Lee
May 1, 2019·Biotechnology and Bioengineering·Moritz K F WolfMassimo Morbidelli
Nov 22, 2011·Biotechnology Journal·Woo Suk Ahn, Maciek R Antoniewicz
Jul 6, 2021·Biotechnology and Bioengineering·Thomas BissingerUdo Reichl

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.