PMID: 7529225Jan 1, 1995Paper

Metabolism of polychlorinated phenols by Pseudomonas cepacia AC1100: determination of the first two steps and specific inhibitory effect of methimazole

Journal of Bacteriology
I TomasiD Mansuy

Abstract

Resting cells of 2,4,5-trichlorophenoxyacetic acid-grown Pseudomonas cepacia AC1100 metabolize both dichlorophenols, such as 2,4-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, and 3,5-dichlorophenol, and more highly substituted phenols, such as 2,4,6-trichlorophenol and pentachlorophenol, to the corresponding chlorohydroquinones. The first hydroxylation occurs in the para position of the phenol regardless of whether this position is replaced by a chlorine substituent. The first evidence leading to the characterization of para-hydroxylase as a flavin-containing enzyme is provided by the inhibitory effect of methimazole, an alternate substrate for this monooxygenase, on the degradative ability of the strain. In a second step, with tetrachlorohydroquinone, trichlorohydroxyquinone was isolated and completely characterized. Trichlorohydroxyquinone was also obtained from tetrachloroquinone. Incubation of the cells in the presence of an external source of NADPH prevents the further degradation of tetrachlorohydroquinone, suggesting that the quinone derived from the two-electron oxidation of the hydroquinone is more likely the substrate for the second hydroxylation.

References

Nov 1, 1979·Archives of Biochemistry and Biophysics·L L PoulsenD M Ziegler
Jan 1, 1991·Biodegradation·L A GolovlevaS Kuzmin
Sep 1, 1992·FEMS Microbiology Reviews·M M Häggblom
Sep 1, 1992·Journal of Bacteriology·J S UotilaM S Salkinoja-Salonen
Jul 1, 1991·Archives of Biochemistry and Biophysics·V RadjendiraneC S Vaidyanathan
Feb 1, 1987·Journal of Bacteriology·J H Apajalahti, M S Salkinoja-Salonen
Nov 1, 1983·Applied and Environmental Microbiology·J S KarnsA M Chakrabarty
Jan 1, 1981·Drug Metabolism Reviews·B Testa, P Jenner
Jul 1, 1982·Applied and Environmental Microbiology·J J KilbaneA M Chakrabarty
Jan 1, 1993·Annual Review of Pharmacology and Toxicology·D M Ziegler
Feb 1, 1989·Applied and Environmental Microbiology·M M HäggblomM S Salkinoja-Salonen

❮ Previous
Next ❯

Citations

Oct 20, 1999·Critical Reviews in Biotechnology·B K SinghK K Tripathi
Nov 9, 2006·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Vasili M TravkinLudmila A Golovleva
Aug 6, 2003·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Naoto OgawaA M Chakrabarty
Jul 5, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Ahmet Çabukİsa Sidir
Apr 25, 2013·PloS One·Aikaterini Papazi, Kiriakos Kotzabasis
Sep 1, 2006·Applied Microbiology and Biotechnology·Arrate JaureguibeitiaJuan L Serra
Jun 1, 2002·Applied and Environmental Microbiology·Alan FrançoisFrédéric Monot
Jul 2, 1998·Applied and Environmental Microbiology·P SteinleK W Hanselmann
Jun 19, 2001·Biochimica Et Biophysica Acta·G Martin-Le GarrecC Capeillère-Blandin
Apr 9, 2010·Journal of Agricultural and Food Chemistry·Young Soo KeumJeong-Han Kim
Jan 22, 1998·Applied and Environmental Microbiology·P PoupinA M Delort
Dec 1, 1995·Applied and Environmental Microbiology·C E DangananA M Chakrabarty

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.