PMID: 2496760Apr 26, 1989Paper

Metabolism of polyunsaturated fatty acids by an (n - 6)-lipoxygenase associated with human ejaculates

Biochimica Et Biophysica Acta
E H Oliw, H W Sprecher

Abstract

Washed cells of normal human ejaculates were incubated with [14C]arachidonic acid (20:4(n - 6] at 37 degrees C for 30-40 min and the main product was characterized as 15(S)-hydroxy-5,8,11,13-eicosatetraenoic acid by reverse phase, straight phase and chiral phase high performance liquid chromatography (HPLC) and by capillary gas chromatography-mass spectrometry. The biosynthesis of 15(S)-hydroxy-5,8,11,13-eicosatetraenoic acid from exogenous 20:4(n - 6) was inhibited by nordihydroguaiaretic acid and abolished by heat inactivation, but it appeared to be unaffected by the ionophore A23187 and Ca2+. Human spermatozoa were partly purified from contaminating material by the swim-up procedure and incubated with 14C-labelled 18:2(n - 6), 20:4(n - 6), 22:5(n - 6) and 22:6(n - 3) for 30-40 min at 37 degrees C. The main radiolabelled products, which were obtained in low yields, co-chromatographed with the Ls (n - 6)-hydroxy fatty acid of each substrate on reverse phase, straight phase and chiral phase HPLC. The (n - 6)-lipoxygenase was also present in ejaculates with oligozoospermia or azoospermia. The seminal fluid contains membrane-surrounded organelles (e.g., 'prostasomes' secreted by the prostate gland) and the (n - 6)-lipoxygenase wa...Continue Reading

References

Jul 1, 1987·Prostaglandins·E H Oliw
Sep 9, 1985·Biochimica Et Biophysica Acta·G Ronquist, I Brody
Jan 1, 1986·Annual Review of Biochemistry·P NeedlemanJ B Lefkowith
Sep 4, 1987·Biochimica Et Biophysica Acta·D H Nugteren, G A Kivits
Jan 1, 1987·Urologia Internationalis·M LindahlG Ronquist
Jun 14, 1985·Biochimica Et Biophysica Acta·M M Milks, H Sprecher
May 15, 1980·Analytical Biochemistry·J M BoeynaemsW C Hubbard
Jan 1, 1981·Prostaglandins·W SchlegelE Nieschlag

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Citations

May 25, 2005·Cell and Tissue Research·S E M Lewis, R J Aitken
Nov 17, 2007·Journal of Assisted Reproduction and Genetics·S M H Andrabi
Dec 1, 1994·European Journal of Obstetrics, Gynecology, and Reproductive Biology·R FabianiG Ronquist
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·A R BrashM S Chang
Dec 1, 1991·Clinical and Experimental Immunology·R W KellyK James
Jan 1, 1993·Acta Physiologica Scandinavica·E H Oliw
Nov 20, 2012·Reproductive Biology and Endocrinology : RB&E·Anil K RenganStefan S du Plessis
May 8, 2000·The Prostate·F G KravetsS A Khan
Mar 3, 2005·Reproduction, Fertility, and Development·R John Aitken
May 28, 1998·The Journal of Biological Chemistry·C Su, E H Oliw
Jul 29, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·K A FischerP Sutovsky

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