Metabolism of prazosin in rat and characterization of metabolites in plasma, urine, faeces, brain and bile using liquid chromatography/mass spectrometry (LC/MS)

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
John C L ErveRasmy E Talaat

Abstract

1. Prazosin, 2-[4-(2-furanoyl)-piperazin-1-yl]-4-amino-6,7-dimethoxyquinazoline, is an antihypertensive agent that has been used safely since 1976 and is currently being investigated for the treatment of post-traumatic stress disorder. The in vivo metabolism of prazosin in rat was first reported in 1977, although at the time analytical techniques were not as sophisticated, nor were the mass spectrometers as sensitive, as today. Recently, the in vitro metabolism of prazosin in rat liver microsomes and cryopreserved hepatocytes was investigated using liquid chromatography/mass spectrometry (LC/MS), which revealed new metabolic pathways. 2. In the present work, rat in vivo metabolism was reinvestigated using a quadrupole time-of-flight mass spectrometer coupled with ultra-performance liquid chromatography, or chip-based nanoflow electrospray ionization, with the aim of identifying metabolites revealed by the in vitro studies and any new metabolites. 3. It is reported that prazosin was metabolized in rats to produce the metabolites observed in vitro. In addition, new phase I metabolites, M18, M20 and M21, were formed and conjugation with glucose or taurine formed the new phase II metabolites, M16 and M19, respectively. 4. Evidence ...Continue Reading

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Citations

Oct 16, 2012·Chemical Research in Toxicology·Lisa A Peterson
Mar 24, 2009·The Analyst·Suwatchai JarussophonYasuo Konishi
Jun 10, 2014·Drug Metabolism Reviews·Jayaprakasam BolleddulaDavid L Bourdet
May 1, 2009·Bioanalysis·Olavi PelkonenHannu Raunio
Mar 4, 2017·Chemical Research in Toxicology·Na Le DangS Joshua Swamidass

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