PMID: 6402034Jan 4, 1983Paper

Metabolism of purine nucleosides in human and ovine lymphocytes and rat thymocytes and their influence on mitogenic stimulation

Biochimica Et Biophysica Acta
G J PetersJ H Veerkamp

Abstract

1. Phosphorolysis and phosphorylation rates of inosine, guanosine and deoxyguanosine were determined in disrupted and intact human and ovine lymphocytes and rat thymocytes and related with their effect on mitogenic stimulation. 2. Activity of purine nucleoside phosphorylase (EC 2.4.2.1) was about 10 times higher in extracts of human lymphocytes than in those of ovine lymphocytes and rat thymocytes. Apparent Km values for inosine and guanosine were higher in human lymphocytes (about 100 microM) than in ovine lymphocytes (50 microM). Apparent Km values for deoxyguanosine were about 100 microM in the extracts of all three cell types. 3. In extracts of human and ovine lymphocytes the presence of guanosine kinase activity was established. Deoxyguanosine kinase activity was detected in all three cell types. 4. The rate of phosphorylation of deoxyguanosine was much lower than the rate of phosphorolysis both in extracts and in intact cells. 5. Deoxyguanosine, guanosine and inosine were incorporated by intact cells into nucleotides and nucleic acids. This incorporation of deoxyguanosine and guanosine was only partially due to phosphorolysis and subsequent conversion by hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8). The inc...Continue Reading

References

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Oct 1, 1981·Canadian Journal of Biochemistry·D HuntingJ F Henderson

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Citations

Sep 1, 1991·Molecular and Biochemical Parasitology·W H Miller, R L Miller
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G J PetersJ H Veerkamp
Sep 23, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A M BergmanG J Peters
May 24, 1997·Biochimica Et Biophysica Acta·F CarlucciA Tabucchi
Jan 1, 1985·Annals of the New York Academy of Sciences·A CohenE W Gelfand
Mar 28, 2017·The Journal of Clinical Investigation·Arimichi OkazakiOthon Iliopoulos
Feb 9, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tingting WengMichael R Blackburn
Sep 2, 2015·Antimicrobial Agents and Chemotherapy·Paul L BoyerStephen H Hughes

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