Metabolism of skin and muscle protein is regulated differently in response to nutrition

The American Journal of Physiology
X J ZhangR R Wolfe

Abstract

We have measured skin and muscle protein kinetics and amino acid (AA) transport in anesthetized rabbits during 1) 64-h fast, 2) AA infusion, 3) AA plus fat emulsion infusion, and 4) AA plus hyperinsulinemia. L-[ring-13C6]phenylalanine was infused as the tracer, and the ear and hindlimb were used as arteriovenous units to reflect skin and muscle protein kinetics, respectively. Skin protein net balance was not different from zero in all groups, indicating a maintenance of protein mass. In contrast, the muscle net balance differed over a range from -1.6 +/- 0.6 after fasting to 0.2 +/- 0.2 mumol.100 g-1.h-1 during hyperinsulinemia. In the skin, 59-66% of intracellular free phenylalanine came from proteolysis, and phenylalanine availability from proteolysis was positively correlated to the protein synthesis rate. In conclusion, normal skin maintains its constant protein mass by efficient reutilization of AAs from proteolysis. In contrast to muscle, skin protein is relatively insensitive to control by nutritional and hormonal factors. Because of the metabolic differences, when limb models are used for muscle protein metabolism, the potential contribution by limb skin should be considered.

References

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Citations

Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·X J ZhangR R Wolfe
Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Xiao-Jun ZhangRobert R Wolfe
Nov 9, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nathalie PoupinHélène Fouillet
Aug 5, 2005·Current Opinion in Clinical Nutrition and Metabolic Care·David L Chinkes
Feb 27, 2003·American Journal of Physiology. Endocrinology and Metabolism·Xiao-Jun ZhangRobert R Wolfe
May 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·Xiao-Jun ZhangRobert R Wolfe
Dec 20, 2007·American Journal of Physiology. Endocrinology and Metabolism·Xiao-Jun ZhangRobert R Wolfe

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