PMID: 3750347Jan 1, 1986Paper

Metabolism of T-2 mycotoxin by cultured cells

Toxicon : Official Journal of the International Society on Toxinology
L R Trusal

Abstract

T-2 mycotoxin is a small (i.e. mol. wt 466), non-protein toxin. We studied its metabolism in Chinese hamster ovary (CHO) cells, African green monkey kidney (VERO) cells, human fibroblasts and mouse connective tissue cells (L-929). Confluent cells were exposed to [3H]-T-2(0.01 micrograms/ml) for 1 hr at 37 degrees C. The toxin was removed, cells rinsed, and unlabeled culture media added for 4 hr (37 degrees C). Cell monolayers were extracted and media and cell extracts were spotted on thin-layer chromatography plates with known standards. Thin-layer plates were developed and scanned for radioactivity, and metabolites were identified based on co-migration with known standards. CHO and VERO cells metabolized T-2 to a greater per cent and to a wider variety of metabolites than the other two cell types. In CHO, fibroblast and L-929 cells, the major metabolite was HT-2 toxin, while in VERO cells an unknown metabolite, more polar than T-2, was the major metabolite. Cell and media extracts of CHO and VERO cells revealed smaller amounts of T-2 triol, T-2 tetraol and several unknowns. In both cell types, metabolites were detected in labeled media by 1 hr and in increasing amounts in unlabeled media by 4 hr. Under the above conditions, 37...Continue Reading

References

Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·L R Trusal, J C O'Brien
Sep 30, 1985·Toxicology and Applied Pharmacology·J G PaceR W Wannemacher

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Citations

Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·W L Thompson, R W Wannemacher
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·P S HoltJ R DeLoach
Jul 1, 1988·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J M PorcherC Frayssinet
May 4, 2012·Journal of Agricultural and Food Chemistry·Maria WeidnerHans-Ulrich Humpf
Feb 1, 2013·Chemical Research in Toxicology·Maria WeidnerHans-Ulrich Humpf
Feb 3, 1989·Journal of Chromatography·H KoshinskyG Khachatourians
Sep 15, 1989·Biochemical Pharmacology·J L Middlebrook, D L Leatherman
Jan 1, 1993·Drug Metabolism Reviews·B Yagen, M Bialer
Sep 1, 1988·Applied and Environmental Microbiology·C J MirochaG Bean
Jul 1, 1991·Applied and Environmental Microbiology·H Babich, E Borenfreund
Aug 19, 1988·Biochimica Et Biophysica Acta·P S Holt, J R DeLoach

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