Metabolism of the extracellular matrix formed by intervertebral disc cells cultured in alginate

Spine
K ChibaE J Thonar

Abstract

Cells from normal rabbit nucleus pulposus (NP) and anulus fibrosus (AF) were cultured in alginate beads for as long as 14 days to allow them to reform a matrix made up of two compartments: the cell-associated matrix (CM) and further removed matrix (FRM). At different time points, the CM and FRM made by each cell population were analyzed using histologic, biochemical, and immunologic assays. To study the metabolism of normal rabbit NP and AF cells in alginate by characterizing the CM and FRM formed by each cell population, and to identify metabolic properties that may shed light on mechanisms at play in disc degeneration. Little is known about the metabolism of intervertebral disc cells, in part because of the lack of microculture systems appropriate for the study of these cells in vitro. In recent studies from our laboratories, it was suggested that articular chondrocytes cultured in alginate beads remain phenotypically stable and reform a matrix similar to the one they populate in vivo. This culture system appears ideally suited for the study of intervertebral cells available only in limited numbers. Rabbit NP and AF cells released from the matrix by sequential enzyme digestion were encapsulated in alginate beads (20,000 cells...Continue Reading

References

Jan 1, 1979·International Review of Connective Tissue Research·D R Eyre
Sep 11, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B A Maldonado, T R Oegema
Apr 1, 1992·Matrix : Collagen and Related Research·H J HäuselmannE J Thonar
Mar 1, 1991·Spine·J P ThompsonD S Bradford
Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Jan 1, 1989·Connective Tissue Research·J F GuoD K MacCallum
Apr 1, 1988·The Anatomical Record·M D Humzah, R W Soames
Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R H PearceM E Adams
Oct 1, 1965·Histochemie. Histochemistry. Histochimie·J E Scott, J Dorling
May 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H OshimaH Tsuji
Sep 1, 1996·The American Journal of Physiology·H J HäuselmannE J Thonar

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Citations

Nov 7, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J W StevensJ A Maynard
Mar 5, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Jun ChenLori A Setton
Dec 5, 2002·Archives of Biochemistry and Biophysics·Hiroshi KamadaEugene J-M A Thonar
May 3, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A E BaerL A Setton
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Y SunR A Kandel
Dec 11, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Masakazu YoshidaAkihiro Matsukawa
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Koichi MasudaEugene J-M A Thonar
Jan 26, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·L GrangR Juvin
Jan 26, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·F RannouS Poiraudeau
Jan 30, 2004·The Spine Journal : Official Journal of the North American Spine Society·Helen E GruberEdward N Hanley
Oct 27, 2009·Nature Materials·Nandan L NerurkarRobert L Mauck
May 24, 2007·Tissue Engineering·Damien M O'Halloran, Abhay S Pandit
Jul 19, 2008·Tissue Engineering. Part a·Alice I ChouSteven B Nicoll
Feb 10, 2011·Tissue Engineering. Part a·Xinlin YangXudong Joshua Li
Jul 9, 2011·Tissue Engineering. Part a·Sang-Hyug ParkDavid L Kaplan
Nov 12, 2009·Tissue Engineering. Part B, Reviews·Koichi Masuda, Jeffrey C Lotz
Nov 27, 2004·Spine·Koichi MasudaHoward S An

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