PMID: 6172599Dec 1, 1981Paper

Metabolism of viral RNA in murine leukemia virus-infected cells; evidence for differential stability of viral message and virion precursor RNA

Journal of Virology
L I MesserS K Chattopadhyay

Abstract

Molecular hybridization techniques were used to examine the stability of viral message and virion precursor RNA in murine leukemia virus-infected cells treated with actinomycin D. Under the conditions used, viral RNA synthesis was inhibited, but viral protein synthesis continued, and the cells produced noninfectious particles (actinomycin D virions) lacking genomic RNA (J. G. Levin and M. J. Rosenak, Proc. Natl. Acad. Sci. U.S.A. 73:1154-1158, 1976). Analysis of total RNA in virions revealed that the amount of hybridizable viral RNA decreased steadily after the addition of actinomycin D and by 8 h was 10% of the control value. Studies on fractionated viral RNA showed that this low level of hybridization is due to residual 70S RNA in the virion population. The results indicated that viral RNA which is destined to be encapsidated into virions has a half-life of approximately 3 to 4 h. In contrast, other intracellular virus-specific RNA molecules appeared to be quite stable and persisted for a long period of time, with a half-life of at least 12 h. These observations support the idea that two independent functional pools of 35S viral RNA exist within the infected cell: one serving as message and the other as precursor to virion RN...Continue Reading

References

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Citations

Mar 9, 2002·Journal of Virology·Melinda Butsch, Kathleen Boris-Lawrie
Oct 28, 2004·Journal of Virology·Jessica A FlynnAlice Telesnitsky
Apr 18, 2014·Journal of Virology·Silas F JohnsonAlice Telesnitsky
Oct 25, 2002·AIDS Research and Human Retroviruses·Chen LiangMark A Wainberg
Jul 15, 2015·RNA Biology·Lucie Pessel-VivaresMarylène Mougel
Apr 15, 2000·The Journal of Biological Chemistry·T OhlmannJ L Darlix
Sep 1, 1993·Journal of Virology·W Fu, A Rein

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