Metabolite exchange within the microbiome produces compounds that influence Drosophila behavior

BioRxiv : the Preprint Server for Biology
Caleb N FischerJo Handelsman

Abstract

Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however current understanding of host-microbiome interactions is derived mostly from studies in which is it is difficult to elucidate microbe-microbe interactions. In exploring how Drosophila melanogaster acquires its microbiome, we found that a microbial community influences Drosophila olfactory and egg-laying behaviors differently than individual members. Drosophila prefers a Saccharomyces - Acetobacter co-culture to the same microorganisms grown individually and then mixed, a response mainly due to the conserved olfactory receptor, Or42b . Acetobacter metabolism of Saccharomyces -derived ethanol was necessary, and acetate and its metabolic derivatives were sufficient, for co-culture preference. Preference correlated with three emergent co-culture properties: ethanol catabolism, a distinct volatile emission profile, and yeast population decline. We describe a molecular mechanism by which a microbial community affects animal behavior. Our results support a model whereby emergent metabolites signal Drosophila to acquire its preferred multispecies microbiome.

Related Concepts

Acetate
Acetobacter
Ethanol
Analogs & derivatives
Drosophila
Drosophila melanogaster
Metabolism
Olfactory Nerve
Saccharomyces
Coculture Techniques

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