Metabolite profiling of dasatinib dosed to Wistar Han rats using automated dried blood spot collection

Journal of Pharmaceutical and Biomedical Analysis
Zhongzhou ShenSadayappan V Rahavendran

Abstract

To characterize and enable efficient rat pharmacokinetic (PK) screening in early drug discovery, automated sampling of blood time points are routinely employed. With the development of dried blood spot (DBS) technology for drug level quantitation, an opportunity exists for the automated collection of rat PK time points using DBS. DBS, as an alternative sample collection technique has led to the increased collection of PK study samples for the quantitative analyses of drug candidates in both pre-clinical and clinical studies. However, the feasibility of using DBS samples for drug metabolite profiling including both phase I and phase II metabolites has not been well established. This work reports the study of metabolite profiling of dasatinib dosed to Wistar Han rats using automated DBS collection. Automated DBS and plasma collection using a rat AccuSampler (VeruTech AB, Sweden) was employed using dasatinib as a model compound. The DBS and plasma samples were extracted by methanol and acetonitrile and both plasma and DBS extracts were analyzed using a Sciex API4000 Qtrap mass spectrometer coupled to a Shimazdzu HPLC system. Dasatinib and its metabolites were analyzed by multiple reaction monitoring (MRM) and MRM trigger enhanced ...Continue Reading

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Sep 25, 2014·Mass Spectrometry Reviews·Michel WagnerGérard Hopfgartner
Apr 3, 2014·Drug Testing and Analysis·Abhisheak SharmaJawahar Lal
Sep 3, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Atsuki MatsubaraMasaru Yoshida
Jan 30, 2019·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Kishore K Katyayan, Yu-Hua Hui
Oct 27, 2021·Bioanalysis·Gangireddy Navitha ReddyRajesh Sonti

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