Metabolites of nucleic acids in bovine milk

Journal of Dairy Science
W TiemeyerD Giesecke

Abstract

To investigate metabolites of nucleic acids in milk as by-products of protein biosynthesis, a method for determination of pyrimidine and purine compounds by reversed-phase high-pressure liquid chromatography was developed. Reproducibility of the measured compounds was 2%. Recovery of the main constituents averaged about 99%. In addition to orotic acid, allantoin, and uric acid, the free bases hypoxanthine, xanthine, and guanine, the ribonucleosides uridine, cytidine, and pseudouridine, and the ribonucleotides, guanosine 5'-monophosphate and cytidine 5'-monophosphate, were quantified in milk samples of Holstein-Friesian cows. Milk production from days 45 to 65 of lactation influenced concentrations of individual metabolites differently. Concentrations of orotic acid, allantoin, and uric acid decreased with increasing milk production, whereas concentrations of uridine, cytidine, pseudouridine, hypoxanthine, xanthine, and guanine remained unchanged. The results allowed conclusions on rate limiting steps of catabolic pathways of pyrimidines and purines and indicated relationships with protein biosynthesis. A possible role of metabolites of nucleic acids as taste factors and as substrates for milk microbes is suggested.

References

May 2, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·A H van GennipS K Wadman
Dec 19, 1973·Biochemical and Biophysical Research Communications·A J VekichB L Larson
Jun 1, 1971·Journal of Dairy Science·M H Chen, B L Larson
Nov 1, 1969·The Journal of Pediatrics·D M BecroftA Simmonds
May 1, 1969·The American Journal of Clinical Nutrition·P O Okonkwo, J E Kinsella
Apr 1, 1982·American Journal of Clinical Pathology·A M Glasgow
Sep 10, 1963·The Journal of Vitaminology·T ADACHIM ASAHINA
Oct 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·L D WRIGHTH R SKEGGS

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Citations

May 25, 2010·Journal of Dairy Science·S PlakantaraE Alichanidis
Feb 1, 1997·Journal of Dairy Science·H L Gonda, J E Lindberg
Apr 22, 2008·The Journal of Dairy Research·Senkiti SakaiKentaro Nagaoka
Mar 10, 2001·The British Journal of Nutrition·E SchlimmeH Meisel
Sep 9, 2010·The Journal of Dairy Research·Torben Larsen, Kasey M Moyes
Jul 12, 2011·Journal of Animal Physiology and Animal Nutrition·G KocicS Zivanovic
Mar 19, 2005·International Journal of Systematic and Evolutionary Microbiology·Lisa D Sprague, Heinrich Neubauer
Jun 4, 2020·Animals : an Open Access Journal From MDPI·Zhongwang LvJiaqi Wang
Dec 29, 2010·Current Rheumatology Reports·Nicola Dalbeth, Kate Palmano
Aug 25, 2009·International Journal of Systematic and Evolutionary Microbiology·B J TindallP Kämpfer
Jun 5, 2016·International Journal of Systematic and Evolutionary Microbiology·Guillaume Ghislain AubinStephane Corvec
Jul 7, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Tsutomu Fujihara, Martin N Shem
Jun 1, 1991·Zeitschrift für Ernährungswissenschaft·E SchlimmeF G Ott
Mar 8, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A TheisingerE Scharrer
Apr 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·K J Shingfield, N W Offer
Mar 18, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·K J Shingfield, N W Offer
Nov 23, 2000·Journal of Agricultural and Food Chemistry·H OstdalJ H Nielsen

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