Metabolites software-assisted flavonoid hunting in plants using ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Wan-Yi GuJian-Lin Wu

Abstract

Plant secondary metabolism drives the generation of metabolites used for host plant resistance, as biopesticides and botanicals, even for the discovery of new therapeutics for human diseases. Flavonoids are one of the largest and most studied classes of specialized plant metabolites. To quickly identify the potential bioactive flavonoids in herbs, a metabolites software-assisted flavonoid hunting approach was developed, which mainly included three steps: firstly, utilizing commercial metabolite software, a flavonoids database was established based on the biosynthetic pathways; secondly, mass spectral data of components in herbs were acquired by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF-MS); and finally, the acquired LC-MS data were imported into the database and the compounds in the herbs were automatically identified by comparison of their mass spectra with the theoretical values. As a case study, the flavonoids in Smilax glabra were profiled using this approach. As a result, 104 flavonoids including 27 potential new compounds were identified. To our knowledge, this is the first report on profiling the components in the plants utilizing the plant metabolic principles ...Continue Reading

References

Jan 25, 2008·Journal of Pharmaceutical and Biomedical Analysis·Lauro M de SouzaGuilherme L Sassaki
Sep 30, 2010·Natural Product Reports·Alok Kumar Verma, Ram Pratap
Feb 15, 2011·Phytochemistry·Boonsong WungsintaweekulHiroshi Noguchi
Jun 30, 2012·Science·Jing-Ke WengJoseph P Noel
Jan 24, 2013·Journal of Agricultural and Food Chemistry·Danny ChanMatthew Sharman
Mar 12, 2013·Plant Physiology and Biochemistry : PPB·Kazuki SaitoAlisdair R Fernie
May 24, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuo XuShao-Qing Cai
Feb 1, 2014·Natural Product Reports·Azra GholamiAlain Goossens
Jul 19, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shao-Dan ChenRui-Zhi Zhao

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Citations

Jun 6, 2018·BMC Complementary and Alternative Medicine·Nadini Oliveira MartinsFúlvio Rieli Mendes
Feb 18, 2018·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Jian LiangHui-Lian Huang
Nov 11, 2020·Current Protein & Peptide Science·Peiyu XueLi Ren

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Methods Mentioned

BETA
MDS
glycosylation

Software Mentioned

MassHunter Metabolite ID
LightSight
Profiler M
metabolites
Agilent MassHunter Metabolite ID
Metabolite
MassHunter
MassHunter Workstation Acquisition
Agilent Metabolite ID

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