Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose

Microbial Cell Factories
Hideo KawaguchiAkihiko Kondo

Abstract

L-Arabinose is the second most abundant component of hemicellulose in lignocellulosic biomass, next to D-xylose. However, few microorganisms are capable of utilizing pentoses, and catabolic genes and operons enabling bacterial utilization of pentoses are typically subject to carbon catabolite repression by more-preferred carbon sources, such as D-glucose, leading to a preferential utilization of D-glucose over pentoses. In order to simultaneously utilize both D-glucose and L-arabinose at the same rate, a modified metabolic pathway was rationally designed based on metabolome analysis. Corynebacterium glutamicum ATCC 31831 utilized D-glucose and L-arabinose simultaneously at a low concentration (3.6 g/L each) but preferentially utilized D-glucose over L-arabinose at a high concentration (15 g/L each), although L-arabinose and D-glucose were consumed at comparable rates in the absence of the second carbon source. Metabolome analysis revealed that phosphofructokinase and pyruvate kinase were major bottlenecks for D-glucose and L-arabinose metabolism, respectively. Based on the results of metabolome analysis, a metabolic pathway was engineered by overexpressing pyruvate kinase in combination with deletion of araR, which encodes a re...Continue Reading

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Citations

Feb 13, 2019·Molecular Microbiology·Gustavo Benevides MartinsMarc Bramkamp
Dec 10, 2019·Biotechnology and Bioengineering·Guan WangSiliang Zhang
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·Xiao-Yu WuBang-Ce Ye
Sep 2, 2019·Trends in Biotechnology·Christopher J VavrickaAkihiko Kondo
Jan 14, 2020·Current Opinion in Biotechnology·Yan ChenChristopher J Petzold

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Methods Mentioned

BETA
phosphotransferase
PCR
Assay

Software Mentioned

Metabolome

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