Metabolome Analysis Identified Okaramines in the Soybean Rhizosphere as a Legacy of Hairy Vetch

Frontiers in Genetics
Nozomu SakuraiAkifumi Sugiyama

Abstract

Inter-organismal communications below ground, such as plant-microbe interactions in the rhizosphere, affect plant growth. Metabolites are shown to play important roles in biological communication, but there still remain a large number of metabolites in soil to be uncovered. Metabolomics, a technique for the comprehensive analysis of metabolites in samples, may uncover the molecules that intermediate these interactions. We conducted a multivariate analysis using liquid chromatography (LC)-mass spectrometry (MS)-based untargeted metabolomics in several soil samples and also targeted metabolome analysis for the identification of the candidate compounds in soil. We identified okaramine A, B, and C in the rhizosphere soil of hairy vetch. Okaramines are indole alkaloids first identified in soybean pulp (okara) inoculated with Penicillium simplicissimum AK-40 and are insecticidal. Okaramine B was detected in the rhizosphere from an open field growing hairy vetch. Okaramine B was also detected in both bulk and rhizosphere soils of soybean grown following hairy vetch, but not detected in soils of soybean without hairy vetch growth. These results suggested that okaramines might be involved in indirect defense of plants against insects. T...Continue Reading

References

May 10, 2003·Journal of Chemical Ecology·Tsunashi KamoYoshiharu Fujii
Jan 18, 2006·The New Phytologist·Robert D Hall
Dec 23, 2008·Journal of Lipid Research·Eoin FahyEdward A Dennis
Feb 1, 2011·Annual Review of Plant Biology·Eran Pichersky, Efraim Lewinsohn
Nov 30, 2011·Plant & Cell Physiology·Farit Mochamad AfendiShigehiko Kanaya
May 9, 2012·Trends in Plant Science·Roeland L BerendsenPeter A H M Bakker
Oct 12, 2012·Nature Biotechnology·Matthew C ChambersParag Mallick
Nov 20, 2012·Nucleic Acids Research·David S WishartAugustin Scalbert
Sep 1, 2007·Metabolomics : Official Journal of the Metabolomic Society·Lloyd W SumnerMark R Viant
Aug 27, 2014·Scientific Reports·Shogo FurutaniKazuhiko Matsuda
Apr 24, 2015·Frontiers in Bioengineering and Biotechnology·Takeshi AraNozomu Sakurai
Aug 4, 2015·Frontiers in Plant Science·Liliana QuizaEtienne Yergeau
Aug 6, 2015·Annual Review of Phytopathology·Barbara Reinhold-HurekThomas Hurek
Oct 18, 2015·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Feb 3, 2016·Trends in Plant Science·Nicole M van Dam, Harro J Bouwmeester
Mar 10, 2018·The ISME Journal·Roeland L BerendsenCorné M J Pieterse
Mar 10, 2018·Cell·Peter A H M BakkerRoeland L Berendsen
Apr 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ioannis A StringlisCorné M J Pieterse
Jun 21, 2018·Frontiers in Plant Science·Qiuying PangSixue Chen
Dec 17, 2018·Current Opinion in Insect Science·Kazuhiko Matsuda
Jun 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Mathias J E E E VogesElizabeth S Sattely

❮ Previous
Next ❯

Citations

Jun 12, 2021·Bioscience, Biotechnology, and Biochemistry·Akifumi Sugiyama

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA

Software Mentioned

LCMS solution
ProteoWizard
PowerGetBatch
TUAT
MFSearcher
Excel
MassChroViewer
Xcalibur

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.