Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng

Journal of Ginseng Research
Guangfei WeiShilin Chen

Abstract

Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants. In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles. There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P < 0.05) in the root tissues of P. quinquefolius and P. notoginseng, respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents ...Continue Reading

Citations

Jun 4, 2021·Microbial Biotechnology·Guangfei WeiShilin Chen
Mar 14, 2020·Critical Reviews in Analytical Chemistry·Jiaqi YueYuanzhong Wang
Aug 14, 2021·Acta Pharmaceutica Sinica. B·Maoqi HouZhengtao Wang

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Methods Mentioned

BETA
PCR
RNA-Seq

Software Mentioned

MEgrey
MetaboAnalyst
BLAST
Tgicl
Bowtie
Trinity
WGCNA
Blast2GO
R package

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