Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats

Journal of Physiology and Biochemistry
Ahmed MedianiN H Lajis

Abstract

Diabetes mellitus (DM) is a chronic disease that can affect metabolism of glucose and other metabolites. In this study, the normal- and obese-diabetic rats were compared to understand the diabetes disorders of type 1 and 2 diabetes mellitus. This was done by evaluating their urine metabolites using proton nuclear magnetic resonance (1H NMR)-based metabolomics and comparing with controls at different time points, considering the induction periods of obesity and diabetes. The biochemical parameters of the serum were also investigated. The obese-diabetic model was developed by feeding the rats a high-fat diet and inducing diabetic conditions with a low dose of streptozotocin (STZ) (25 mg/kg bw). However, the normal rats were induced by a high dose of STZ (55 mg/kg bw). A partial least squares discriminant analysis (PLS-DA) model showed the biomarkers of both DM types compared to control. The synthesis and degradation of ketone bodies, tricarboxylic (TCA) cycles, and amino acid pathways were the ones most involved in the variation with the highest impact. The diabetic groups also exhibited a noticeable increase in the plasma glucose level and lipid profile disorders compared to the control. There was also an increase in the plasma ...Continue Reading

References

Jun 28, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·K SrinivasanP Ramarao
Dec 28, 2006·Physiological Genomics·R M SalekJ L Griffin
Jan 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Vijay SharmaJohn H McNeill
Aug 16, 2008·Journal of Agricultural and Food Chemistry·Abbe Maleyki Mhd JalilSyed Hasbullah Syed Kamaruddin
Oct 31, 2009·Diabetes·James R BainChristopher B Newgard
Sep 3, 2010·Diabetes/metabolism Research and Reviews·Jun LiuLuyong Zhang
Sep 25, 2010·European Journal of Pharmacology·Vilapakkam Ranganathan PunithavathiJemmi Selvakumari
Jan 26, 2012·Diabetes Care·Jennifer A HirstRichard J Stevens
Oct 15, 2014·Saudi Journal of Biological Sciences·I B Abdel-FaridE A Mohamed
Apr 22, 2015·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Apr 29, 2015·Molecular BioSystems·Mohamad Hafizi Abu BakarHarisun Yaakob
Jun 16, 2015·Molecules and Cells·Seokjae ParkEun-Kyoung Kim
Jun 22, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lili SongYanjun Zhang
Jan 18, 2016·Journal of Ethnopharmacology· MaulidianiN H Lajis

❮ Previous
Next ❯

Citations

Nov 14, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qian GuoLirong Wu
Nov 25, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mengjun XieMei Zhang
May 26, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Maede HasanpourMehrdad Iranshahi
May 15, 2021·Applied Spectroscopy·Nicole M Ralbovsky, Igor K Lednev
Aug 15, 2021·Metabolomics : Official Journal of the Metabolomic Society·Catarina Luís SilvaJosé S Câmara

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
urine collection
blood collection
PCA

Software Mentioned

LifeScan
Simca
MetPA
DA
MetaboAnalyst
METLIN
GraphPad
InStat
Chenomx NMR Suite
MVDA

Related Concepts

Related Feeds

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.