Metabolomic analysis of acid stress response in Saccharomyces cerevisiae

Journal of Bioscience and Bioengineering
Riyanto Heru NugrohoHiroshi Shimizu

Abstract

Acid stress has been reported to inhibit cell growth and decrease productivity during bio-production processes. In this study, a metabolomics approach was conducted to understand the effect of lactic acid induced stress on metabolite pools in Saccharomyces cerevisiae. Cells were cultured with lactic acid as the acidulant, with or without initial pH control, i.e., at pH 6 or pH 2.5, respectively. Under conditions of low pH, lactic acid led to a decrease in the intracellular pH and specific growth rate; however, these parameters remained unaltered in the cultures with pH control. Capillary electrophoresis-mass spectrometry followed by a statistical principal component analysis was used to identify the metabolites and measure the increased concentrations of ATP, glutathione and proline during severe acid stress. Addition of proline to the acidified cultures improved the specific growth rates. We hypothesized that addition of proline protected the cells from acid stress by combating acid-induced oxidative stress. Lactic acid diffusion into the cell resulted in intracellular acidification, which elicited an oxidative stress response and resulted in increased glutathione levels.

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Citations

Jun 22, 2016·Nucleic Acids Research·Arun S RajkumarJay D Keasling
Nov 1, 2016·Electrophoresis·Rawi RamautarGerhardus J de Jong
Sep 4, 2019·Journal of Food Science and Technology·Mariana S LinguaMaría V Baroni
Apr 14, 2017·World Journal of Microbiology & Biotechnology·Peng GengGui Yang Shi
Aug 15, 2017·Electrophoresis·Véréna PoinsotFrançois Couderc
Nov 7, 2020·Frontiers in Bioengineering and Biotechnology·Eugene Fletcher, Kristin Baetz
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc
Oct 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Yuanyuan PanDa-Wen Sun

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