Metabolomic analysis of mammalian cells and human tissue through one-pot two stage derivatizations using sheathless capillary electrophoresis-electrospray ionization-mass spectrometry

Journal of Chromatography. a
Tianjiao HuangJames L Edwards

Abstract

Analysis of metabolites is often performed using separations coupled to mass spectrometry which is challenging due to their vast structural heterogeneity and variable charge states. Metabolites are often separated based on their class/functional group which in large part determine their acidity or basicity. This charge state dictates the ionization mode and efficiency of the molecule. To improve the sensitivity and expand the coverage of the mammalian metabolome, multifunctional derivatization with sheathless CE-ESI-MS was undertaken. In this work, amines, hydroxyls and carboxylates were labeled with tertiary amines tags. This derivatization was performed in under 100 min and resulted in high positive charge states for all analytes investigated. Amino acids and organic acids showed average limits of detection of 76 nM with good linearity of 0.96 and 10% RSD for peak area. Applying this metabolomic profiling system to bovine aortic endothelial cells showed changes in 15 metabolites after treatment with high glucose. The sample injection volume on-capillary was <300 cells for quantitative analyses. Targeted metabolites were found in human tissue, which indicates possible application of the system complex metabolome quantitation.

Citations

Sep 10, 2020·Electrophoresis·Wei Zhang, Rawi Ramautar
Feb 10, 2021·Critical Reviews in Analytical Chemistry·Vladimir G Zaikin, Roman S Borisov
Apr 18, 2019·Journal of the American Society for Mass Spectrometry·Tianjiao HuangJames L Edwards
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc
Jan 2, 2021·Analytica Chimica Acta·Renmeng Liu, Zhibo Yang
Dec 16, 2020·ACS Biomaterials Science & Engineering·Tianjiao HuangChengpeng Chen
Oct 19, 2019·Analytical Chemistry·Carolina González-RianoCoral Barbas
Nov 9, 2019·Analytical Chemistry·Courtney J KristoffLisa A Holland
Nov 6, 2018·Analytical Chemistry·Tianjiao HuangJames L Edwards

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