Metabolomic Analysis of the Urine from Rats with Collagen-Induced Arthritis with the Effective Part of Caulophyllum robustum Maxim.

Evidence-based Complementary and Alternative Medicine : ECAM
Shaowa LüHaixue Kuang

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease with high incidence and high disability and recurrence rates. Caulophyllum robustum Maxim (C. robustum) is a traditional Chinese medicine (TCM) with main effective parts (CRME) commonly used for RA treatment. To explore the mechanism of CRME in RA, we used metabolomics to investigate the effect of CRME intervention on urine metabolism in rats with collagen-induced arthritis (CIA). CIA rats were randomly divided into normal control, CIA model, and CRME groups. A metabolomics approach, using Ultra-Performance Liquid Chromatography-Quadrupole-Time-of-Flight/Mass Spectrometry, was developed to perform urinary metabolic profiling. Differential metabolites were identified by comparing the CIA model and CRME groups. Preliminarily, 56 significant differential metabolites were identified in urine, and 20 metabolic pathways were disturbed by the CIA. The amount of 16 different metabolites changed in urine after CRME intervention. The production of these metabolites involves tryptophan, tyrosine, energy, cholesterol, and vitamin metabolism. CRME has anti-inflammatory and immunosuppressive effects in CIA model rats. By examining the endogenous metabolite levels, we identified potent...Continue Reading

References

Jan 1, 2008·Antiviral Research·A FraternaleM Magnani
Aug 21, 2010·Journal of Pharmacological Sciences·Kaiwei SiXiaoqi Li
Oct 20, 2015·Journal of Pharmaceutical and Biomedical Analysis·Adam ZabekPiotr Mlynarz
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Oct 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shaowa LüQiuhong Wang

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