Metabolomic analysis of uterine serous carcinoma with acquired resistance to paclitaxel.

Oncotarget
Manabu SeinoSatoru Nagase

Abstract

Uterine serous carcinoma (USC) is more aggressive than other subtypes of endometrial carcinoma and is associated with a poor prognosis. We analyzed the metabolomic profile of USC with acquired resistance to paclitaxel. Glutathione (GSH) concentration in PTX-1 cells was higher than in USPC-1 cells. In addition, GSH concentration in the USPC-1 cells increased after treatment with paclitaxel but was unchanged in PTX-1 cells. Glucose-6-phosphate (G6P) and ribose-5-phosphate (R5P) concentrations in PTX-1 cells were higher than those in USPC-1 cells. G6P concentration in the USPC-1 cells was unchanged after treatment with paclitaxel, while it decreased in PTX-1 cells. Our results indicate that increased GSH and glucose metabolism may be related to acquiring resistance to paclitaxel in USC and thus may be targets for anti-USC therapy. We compared metabolic profiles and reactions to paclitaxel in both a wild type USC cell line (USPC-1) and PTX-1, a cell line derived from USPC-1 which acquired paclitaxel resistance, using a capillary electrophoresis CE-MS/MS system.

References

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Citations

Sep 10, 2020·Electrophoresis·Wei Zhang, Rawi Ramautar

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Methods Mentioned

BETA
metabolomic profiling
electrophoresis
Assay
protein assay

Software Mentioned

MasterHands
FlowJo
MassHunter Quantitative Analysis

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