Metabolomic analysis reveals distinct profiles in the plasma and urine of rats fed a high-protein diet

Amino Acids
Chunlong MuWeiyun Zhu

Abstract

A high-protein, low-carbohydrate diet has been regarded as a dietary intervention for weight loss in the obese population. We integrated metabolomics profiles and correlation-based network analysis to reveal the difference in metabolism under diets with different protein:carbohydrate ratios. Rats were fed a control diet (moderate-protein moderate-carbohydrate: MPMC; 20 % protein, 56 % carbohydrate) or HPLC diet (high-protein low-carbohydrate: 45 % protein, 30 % carbohydrate) for 6 weeks. The fat content was equal for both diets. HPLC feeding induced weight loss and reduced adipose weight and plasma triglyceride. Compared to the MPMC diet, HPLC significantly increased plasma α-tocopherol, pyruvate, 2-oxoisocaproate, and β-hydroxybutyrate, and reduced linoleate, palmitate, α-glycerophosphate and pyroglutamic acid. The HPLC-associated urinary metabolite profile was signified with an increase in palmitate and stearate and a reduction of citrate, 2-ketoglutarate, malate, and pantothenate. Pathway analysis implicated a significant alteration of the TCA cycle in urine. Biomarker screening demonstrated that individual metabolites, including plasma urea, pyruvate, and urinary citrate, robustly distinguished the HPLC group from the MPMC ...Continue Reading

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Citations

Aug 20, 2015·The British Journal of Nutrition·Catherine ChaumontetDaniel Tomé
Nov 4, 2016·Amino Acids·Antonio Herbert LanchaFrancois Blachier
Jan 30, 2016·Briefings in Bioinformatics·Clément Frainay, Fabien Jourdan
Sep 2, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Magdalena StepienClaire Gaudichon

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