Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts.

Proceedings of the National Academy of Sciences of the United States of America
Rui WuLe Kang

Abstract

Phenotypic plasticity occurs prevalently and plays a vital role in adaptive evolution. However, the underlying molecular mechanisms responsible for the expression of alternate phenotypes remain unknown. Here, a density-dependent phase polyphenism of Locusta migratoria was used as the study model to identify key signaling molecules regulating the expression of phenotypic plasticity. Metabolomic analysis, using high-performance liquid chromatography and gas chromatography-mass spectrometry, showed that solitarious and gregarious locusts have distinct metabolic profiles in hemolymph. A total of 319 metabolites, many of which are involved in lipid metabolism, differed significantly in concentration between the phases. In addition, the time course of changes in the metabolic profiles of locust hemolymph that accompany phase transition was analyzed. Carnitine and its acyl derivatives, which are involved in the lipid β-oxidation process, were identified as key differential metabolites that display robust correlation with the time courses of phase transition. RNAi silencing of two key enzymes from the carnitine system, carnitine acetyltransferase and palmitoyltransferase, resulted in a behavioral transition from the gregarious to solit...Continue Reading

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Citations

Aug 31, 2013·Omics : a Journal of Integrative Biology·Aihua ZhangXijun Wang
Jan 16, 2014·Nature Communications·Xianhui WangLe Kang
Oct 29, 2013·Annual Review of Entomology·Xianhui Wang, Le Kang
May 20, 2014·Applied Biochemistry and Biotechnology·Aihua ZhangXijun Wang
Jan 6, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jing HeLe Kang
Apr 1, 2015·Entomologia Experimentalis Et Applicata·Charles J P SnartDavid A Barrett
Oct 20, 2015·The American Journal of Chinese Medicine·Pengcheng WangHaixue Kuang
Dec 3, 2014·Insect Biochemistry and Molecular Biology·Qianquan ChenLe Kang
Dec 24, 2014·Insect Biochemistry and Molecular Biology·Quanmei ChenQingyou Xia
Apr 16, 2015·Frontiers in Behavioral Neuroscience·Xiaojiao GuoLe Kang
May 15, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ai-hua ZhangXi-jun Wang
Jan 15, 2015·Omics : a Journal of Integrative Biology·Hui SunXijun Wang
Jun 28, 2016·Insect Biochemistry and Molecular Biology·Siyuan GuoLe Kang
Dec 18, 2015·Journal of the Science of Food and Agriculture·Jian LiuJia-Shun Gong
Oct 8, 2016·Scientific Reports·Apostolos KapranasDavid A Barrett
Jul 23, 2014·Journal of Proteome Research·Maoqing WangChanghao Sun
Jan 19, 2018·Scientific Reports·Bernard W M WoneBeate Wone
Jul 12, 2019·Protein & Cell·Pengcheng YangLe Kang
Nov 9, 2019·The Journal of Biological Chemistry·Vytas A Bankaitis, Zhigang Xie
Mar 4, 2020·Molecular and Cellular Biology·Cory J WhiteMichael J Wolfgang
Aug 29, 2020·Journal of Photochemistry and Photobiology. B, Biology·Xiaolong GaoCaihuan Ke

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